|
|||||
|
|
| Detection of HBV and HCV Coinfection by TEM with Au Nanoparticle Gene Probes | |
| 作者姓名： | 习东 罗小平 宁琴 |
| 作者单位： | Laboratory of Infectious Immunology Huazhong University of Science and Technology，Department of Pediatrics，Huazhong University of Science and Technology，Laboratory of Infectious Immunology Huazhong University of Science and Technology，Wuhan 430030 China，Wuhan 430030 China，Wuhan 430030 China Department of Infectious Disease Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China |
| 摘 要： | Gold(Au) nanoparticle HBV DNA or HCV cDNA gene probes were prepared and were used to detect HBV DNA and HCV RNA extracted from positive serum of patients with HBV and HCV coinfection directly by transmission electron microscopy(TEM).PCR identifying HBV and HCV in serum of patients with HBV and HCV coinfection was established.Alkanethiol-modified oligonucleotide was bound with self-made Au nanoparticles to form nanoparticle HBV DNA or HCV cDNA gene probes through covalent binding of Au-S.HBV DNA and HCV RNA extracted from positive serum of patients with HBV and HCV coinfection was added to the detection system composed of nanoparticle HBV DNA and(or) HCV cDNA gene probes.The results showed that HBV DNA and HCV RNA could be specifically amplified by PCR.The zones of DNA amplification appeared in 431 bp and 323 bp respectively.When HBV DNA and HCV RNA extracted from positive serum of patients with HBV and HCV coinfection were added to the detection system,TEM displayed the nanoparticles self-assembled into large network aggregates.It was concluded that the detection of HBV and HCV coinfection by TEM was convenient and efficient with high specificity and sensitivity. |
| 关 键 词： | 乙型肝炎病毒 丙型肝炎病毒 联合感染 金纳米颗粒基因探针 |
| 收稿时间： | 2007-03-12 |
Detection of HBV and HCV coinfection by TEM with Au nanoparticle gene probes |
|
| Xi?Dong,Luo?Xiaoping,Ning?Qin.Detection of HBV and HCV Coinfection by TEM with Au Nanoparticle Gene Probes[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2007,27(5):532-534. | |
| Authors: | Xi Dong Luo Xiaoping Ning Qin |
| Institution: | 1. Laboratory of Infectious Immunology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China 2. Department of Pediatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China 3. Laboratory of Infectious Immunology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;Department of Infectious Disease, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China |
| Abstract: | Gold(Au) nanoparticle HBV DNA or HCV cDNA gene probes were prepared and were used to detect HBV DNA and HCV RNA extracted from positive serum of patients with HBV and HCV coinfection directly by transmission electron microscopy(TEM).PCR identifying HBV and HCV in serum of patients with HBV and HCV coinfection was established.Alkanethiol-modified oligonucleotide was bound with self-made Au nanoparticles to form nanoparticle HBV DNA or HCV cDNA gene probes through covalent binding of Au-S.HBV DNA and HCV RNA extracted from positive serum of patients with HBV and HCV coinfection was added to the detection system composed of nanoparticle HBV DNA and(or) HCV cDNA gene probes.The results showed that HBV DNA and HCV RNA could be specifically amplified by PCR.The zones of DNA amplification appeared in 431 bp and 323 bp respectively.When HBV DNA and HCV RNA extracted from positive serum of patients with HBV and HCV coinfection were added to the detection system,TEM displayed the nanoparticles self-assembled into large network aggregates.It was concluded that the detection of HBV and HCV coinfection by TEM was convenient and efficient with high specificity and sensitivity. |
| Keywords: | hepatitis B virus hepatitis C Au nanoparticles DNA probes |
| 本文献已被 CNKI 维普 万方数据 SpringerLink 等数据库收录！ | |