幽门螺杆菌相关的消化性溃疡患儿细胞免疫功能研究

目的检测42例幽门螺杆菌（HP）感染相关的消化性溃疡外周血白细胞介素-2（IL-2）、可溶性白细胞介素2受体（sIL-2R）、白细胞介素6和8（IL-6和IL-8）和T淋巴细胞亚群,以探讨其免疫学发病机制。方法IL-2、sIL-2R、IL-6、IL-8和T淋巴细胞亚群检测,分别采用ELISA、双抗体夹心ELISA和APAAP法进行。结果CD3＋、CD4＋细胞百分率、CD4＋/CD8＋比值和IL-2水平均显著低于对照组（P均〈0.01）,而CD8＋细胞百分率、sIL-2R、IL-6、IL-8水平均显著高于对照组（P均〈0.01）。结论HP感染相关的消化性溃疡患儿细胞免疫功能低下且紊乱,该患儿机体的免疫功能障碍在该病的发生中起一定作用。

Study on cellular immunity in childhood peptic ulcer disease correlated with helicobacter pylori.

Objective：To examine interleukin-8.6.2（IL-8.6.2）,serum soluble IL-2 receptor（sIL-2R） levels and T lymphocyte subsets in peripheral blood of 42 childhood peptic ulcer disease correlated with helicobacter pylori（HP） and analyse its immnunological pathogenesis.Methods：IL-8 and IL-6,IL-2 and sIL-2R as well as T lymphocyte subsets were assayed by double antibody sandwich ELISA,ELISA and APAAP Methods.Results：The percentages of CD3＋ and CD4＋ and the ratio of CD4＋/ CD8＋ cells as well as IL-2 level in the patients were significantly lower ﹙P〈0.01,respectively,while sIL-2R,IL-6 and IL-8 levels were higher than those in normal controls ﹙P〈0.01,respectively﹚,respectively.Conclusion：Cellular immunity function is low and disorder in childhood peptic ulcer disease correlated with HP and this disorders in cellular immunity function have some effect in its pathogenesis.