淫羊藿素对大鼠软骨细胞作用的实验研究

目的：研究淫羊藿素对大鼠软骨细胞增殖、凋亡的影响。方法：将细胞随机分为6组，分别予以浓度为0，4，8，16，32，64 μmol/L的淫羊藿素(纯度为99%)处理不同时间组。药物作用于细胞后，MTT比色法检测大鼠软骨细胞的增殖，Annexin V/PI双染色流式细胞术检测细胞的凋亡。结果：MTT 实验结果显示，4和8 μmol/L的淫羊藿素作用于大鼠软骨细胞72 h，大鼠软骨细胞的增殖能力高于对照组(P<0.05)；16，32，64 μmol/L的淫羊藿素作用于大鼠软骨细胞72 h，大鼠软骨细胞的增殖能力低于对照组(P<0.05)。流式细胞仪检测结果显示，在药物处理24 h和48 h后，4 μmol/L的淫羊藿素所引起的细胞凋亡率较对照组明显降低(P<0.05)；8，16，32 μmol/L的淫羊藿素则会增加细胞的凋亡率(P<0.05)。结论：淫羊藿素在低浓度(4，8 μmol/L)时能促进大鼠软骨细胞的体外增殖和抑制凋亡，而过高浓度的淫羊藿素反会抑制细胞增殖和促进凋亡。

A study on the effect of icaritin on rat chondrocytes

Objective: To investigate the eff ect of icaritin on proliferation and apoptosis in rat chondrocytesand to provide new theory for osteochondropathy treatment.Methods: Icaritin (with a purity of 99%) at diff erent concentrations (0, 4, 8, 16, 32, 64 μmol/L)was incubated with rat chondrocytes for different time. The cell proliferation and apoptosis wasassayed by MTT and fl ow cytometry, respectively.Results: Compared with the control group, the cell proliferation was increased in the groups withicaritin at 4 or 8 μmol/L (P<0.05), whereas the proliferation was decreased in the groups withicaritin at 16, 32 or 64 μmol/L groups compared to the control group (P<0.05); the cell apoptosisratio in the group with icaritin at 4 μmol/L was obviously lower than that in the control group aft erincubation of icaritin for 24 h and 48 h. Beyond 4 μmol/L, the higher concentration of icaritin,the higher apoptosis ratio of cell. However, it did not show a time-dependent manner at a sameconcentration of icaritin.Conclusion: The icaritin at low concentration (4 or 8 μmol/L) can promote rat chondrocyteproliferation and inhibit cell apoptosis, while the effect of icaritin on rat chondrocyte at highconcentration was reversed.