MiR-149 基因多态性与类风湿性关节炎的相关性

目的：探讨微小RNA(microRNA，miRNA/miR)-149单核苷酸多态性与华东地区汉族人群类风湿性关节炎的关联性。方法：200例类风湿性关节炎患者与120例健康对照均来源于南京市第一医院风湿免疫科2010-2014年的门诊和住院患者。在获取研究对象知情同意后，收集其临床资料并抽取2 mL外周静脉血，提取基因组DNA。利用限制性片段长度多态性聚合酶链反应(polymerase chain reaction-restriction fragment length polymorphism，PCR-RFLP)技术对研究对象miR-149 rs22928323的单核苷酸多态性进行检测。比较两组对象相关临床参数与单核苷酸多态性的相关性。结果：类风湿性关节炎患者与健康对照组miR-149 rs22928323的3种基因型TT ，TC，CC分别为25.3%，51.1%，23.6%和18.3%，20.0%，61.7%；与miR-149基因型TT +TC相比，基因型为CC的患者发病风险增加[OR=1.38，95%CI(1.01~1.75)，P=0.023]。RA患者miR-149各基因型间类风湿因子、肌苷、抗角蛋白抗体等临床参数比较，差异无统计学意义(P>0.05)。结论：MiR-149的单核苷酸多态性位点rs22928323与华东地区汉族人群类风湿关节炎的遗传易感性相关；其多态性与RA 患者相关临床参数间没有相关性。

Single nucleotide polymorphism of miR-149 and susceptibility of rheumatoid arthritis

Objective: To investigate the association between microRNA (miR)-149 polymorphism andsusceptibility to rheumatoid arthritis (RA ), as well as the clinical characteristics in patients with RA .Methods: A total of 200 RA patients and 120 healthy controls were recruited from Departmentof Rheumatology and Immunology of Nanjing First Hospital. After obtaining the informedconsent, we collected 2 mL of anti-coagulated venous blood samples from all studied subjectsto isolate the whole blood genomic DNA, and the clinical data were collected as well. Singlenucleotide polymorphisms of miR-149 rs22928323 were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Correlation between single nucleotidepolymorphisms and clinical features were compared.Results: The frequencies of TT, TC and CC for rs22928323 of miR-149 were 25.3%, 51.1% and 23.6%or 18.3%, 20.0% and 61.7% in the patients or the healthy controls, respectively. The onset risk of alleleC in RA patients was increased compared with allele T [OR=1.38, 95% CI (1.01–1.75), P=0.023].There were no significant difference in rheumatoid factor, blood urine nitrogen, antikeratin antibody,and other clinical characteristics among the 3 genotypes in RA patients (P>0.05).Conclusion: SNP rs22928323 in miR-149 is correlated with RA in the east of Chinese Hanpopulation, whereas there is no correlation between miR-149 polymorphism and clinicalcharacteristics in patients with RA.