茵陈五苓散对梗阻性黄疸大鼠肝组织中钙蛋白酶的干预作用

目的：探索梗阻性黄疸时钙蛋白酶在大鼠肝组织中的表达变化及其意义；探讨梗阻性黄疸术后中药茵陈 五苓散对肝脏的保护作用及与钙蛋白酶表达的关系。方法：建立梗阻性黄疸SD大鼠肝损害模型，并将成年SD大鼠分 为假手术对照组(S组)，梗阻性黄疸组(O组)，梗阻性黄疸+中药茵陈五苓散组(OC组)，梗阻性黄疸+生理盐水组(OW 组)。检测血清中TBIL，ALT，AST等生化指标含量；肝组织切片行HE染色观察肝组织的病理改变；免疫组织化学 和Western印迹测肝组织中钙蛋白酶表达变化；Real-time PCR检测大鼠肝组织钙蛋白酶基因表达量，观察梗阻性黄疸 时钙蛋白酶在大鼠肝组织中的表达变化。结果：随着造模时间的延长，钙蛋白酶蛋白及基因表达水平呈逐渐增加趋 势。梗阻性黄疸大鼠给予中药茵陈五苓散可以抑制钙蛋白酶的表达，减少因钙蛋白酶高表达引起的肝细胞损伤，改 善肝脏因梗阻引起的肝功能异常。钙蛋白酶基因表达水平与梗阻性黄疸肝损伤程度变化一致。结论：梗阻性黄疸患 者辩证使用茵陈五苓散可促进术后肝脏功能恢复，降低并发症发生率，从而为临床围手术期合理应用中药提供理论 依据。

Effect of artenisiae scopariae and poriae powder on calpain-2 expression in liver tissue from rats with obstructive jaundice

Objective: To explore the eff ect of artenisiae scopariae and poriae powder (ASPD) on calpain-2 expression in liver tissue from rats with obstructive jaundice. Methods: The rat model of obstructive jaundice was established. SD rats was divided into the control group, the obstructive jaundice group, the obstructive jaundice model plus ASPD group, the obstructive jaundice model plus saline group. Th e serum levels of TBIL, ALT, AST and other biochemical indexes were detected. The pathological changes of liver tissue were evaluated by HE staining. The calpain-2 mRNA and protein expression in liver was measured by Real-time PCR and immunohistochemistry or Western blot, respectively. Results: The calpain-2 mRNA and protein expression levels were significantly up-regulated in live tissues from the rats with obstructive jaundice in a time-dependent manner. The ASPD could inhibit the calpain-2 expression in rats with obstructive jaundice concomitant with the decreased liver damage and the improved liver function, suggesting that calpain-2 was involved in endoplasmic reticulum stress-mediated cellular apoptosis and the occurrence of obstructive jaundice. Conclusion: ASPD could be used for patients with obstructive jaundice to promote the recovery of liver function after operation and to reduce the incidence of complications, which provide a theoretical basis for the reasonable application of traditional Chinese medicine in the peroperative period.