MMP-9和TIMP-1在不明原因不孕者子宫内膜的表达

目的检测基质金属蛋白酶-9（MMP-9）和组织金属蛋白酶抑制物-1（TIMP-1）在不明原因不孕、正常早孕蜕膜组织和正常子宫内膜中的表达，探讨其与不明原因不孕关系。方法应用免疫组化S—P法，检测MMP-9和TIMP-1在30例不明原因不孕症患者的种植窗期子宫内膜、20例早孕蜕膜组织和20例正常子宫内膜中的表达情况。结果MMP-9和TIMP-1在宫内早孕蜕膜组织表达程度最高，在正常予宫内膜中表达较高，而在不明原因不孕子宫内膜表达水平显著降低，MMP-9和TIMP-1在三组之间比较，差异均有统计学意义（P均〈0．05）。结论MMP-9和TIMP—I在不明原因不孕子宫内膜中呈低水平表达，且无分泌期表达水平的增强，这可能会导致子宫内膜接受性的降低，使着床失败引起不孕。

The expression of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 protein in cycling endometrium of patients with primary unexplained infertility

Objective To detect the expression of matrixmetalloproteinase-9(MMP-9) and tissue inhibitor of metalloproteinase-1 protein(TIMP-1) in cycling endometrium of patients with unexplained infertility,normal endometrium and normal early pregnant deciduas and explore the relationship between the expression of MMP-9 and TIMP-1 in unexplained infertility and normal pregancy.Methods Immunohistochememstry(S-P technique) and pathologic image semi-quantification were used to detect the expression of MMP-9 and TIMP-1 in endometrium of 30 unexplained infertility patients and 20 normal early pregnant deciduas and 20 normal endometrium.Results The expression of MMP-9 and TIMP-1 in normal early pregnant deciduas was the highest,showing significant difference as compared with other two groups(P<0.05) The expression of MMP-9 and TIMP-1 was also high in normal endometrium as compared with other two groups(P<0.05).But the expression of MMP-9 and TIMP-1 in endometrium of patients with unexplained infertility was significantly reduced as compared with other two groups(P<0.05).Conclusion In unexplained infertility women,the low expression of MMP-9 and TIM P-1 protein in the implantation window phase may cause the failure of implantation and results in the infertility.