外膜袖套吻合法修复雌性SD大白鼠坐骨神经离断的实验研究

目的 通过动物实验,比较神经外膜袖套吻合法和神经外膜直接吻合法修复离断周围神经的效果.方法 雌性成年SD大白鼠48只,随机分为两组,即神经外膜直接吻合法组(对照组)、神经外膜袖套吻合法组(实验组),每组24只.两组皆解剖出左侧坐骨神经并与中段横行离断,对照组以神经外膜直接吻合法吻合离断神经；实验组以神经外膜袖套吻合法吻...

Experimental study of repairing transection of the female sd rats' sciatic nerve by epineurial cuff anastomosis method

【Objective】 Through animal experiment,comparing the effect on repairing of peripheral nerve between epineurial cuff anastomosis method group and direct epineurial anastomosis method.【Methods】 48 female SD rats,randomly divided into 2 experiment groups,that is epineurial cuff anastomosis method group（experiment group）,direct epineurial anastomosis method group（control group）,each group had 24 rats.Every rats of each group were mid-thigh sciatic Nerve,transverse cut,which is in control group the broken nerve was sutured directly,in experiment group sutured by epineurial small gap anastomosis method.At 4,8,12 weeks post-op,took out 8 rats from each groups,examined the anastomotic pattern under microscope,histological observation,Myelinated fiber counts of sciatic nerve,gastrocnemius muscle wet weight gastrocnemius＇ biggest contracture,made a statistical analysis.【Results】 In all observation time,Control group,post-op,the adhesion between the anastomosis site and the surrounding tissue was severer,scar tissue proliferates,and there was neuroma formation;Experiment group,no obvious adhesion formation can be seen,mild scar tissue proliferation can be seen around the venous bridge,,and no neuroma formation.From total myelinated nerve fibers of each group：At 12 weeks,the number of Control group is bigger than Experiment group.p0.05,the difference was statistically significant.The wet weight of the gastrocnemius muscle：at 8 weeks,Experiment group is heavier,p 0.05,the difference was statistically significant.The response rate of the maximum tetanic contraction： at 12 weeks,Experiment group is heavier,p 0.05,the difference was statistically significant.【Conclusion】 the effect on repairing of peripheral nerve by Epineurial cuff anastomosis is better than by the epineurium direct anastomosis.