CXCR1/CXCR2受体拮抗剂G31P对前列腺癌细胞增殖和转移的抑制作用

目的探讨CXCR1/CXCR2受体拮抗剂G31P体外对人前列腺癌PC-3细胞增殖和转移的抑制作用。方法以不同浓度的G31P作用于人前列腺癌PC-3细胞,采用CCK-8法、ECM基质粘附实验和Transwell小室侵袭实验研究G31P对PC-3细胞生长、粘附和转移的抑制作用。结果 CCK-8法检测结果显示100ng/mL G31P作用1、3、5d可抑制PC-3细胞的增殖,与对照组(0ng/mL)相比差异均具有统计学意义(P<0.01,P<0.05)。1ng/mL和100ng/mL G31P预处理1d的细胞进行ECM基质粘附实验,其细胞粘附率均明显低于0ng/mL组(均P<0.01)。Transwell小室侵袭实验结果显示,0、1和100ng/mL G31P处理组平均每个400倍视野下的穿膜细胞数分别为(35±6)、(33±4)和(25±4)个,其中100ng/mL G31P处理组明显低于同时点的0ng/mL组(P<0.05)。结论 G31P在体外实验中能抑制雄激素非依赖性前列腺癌细胞系PC-3的增殖、粘附和转移。

Inhibitory Effects of G31P on Proliferation and Metastasis of Human Prostate Cancer Cells

Objective To investigate the effects of G31P on the proliferation,adhesion and metastasis characteristics of the prostate cancer cells in vitro.Methods The inhibitory effects of G31P on growth,adhesion and metastasis of PC-3 cells were investigated respectively by using CCK-8,ECM matrix assay and Transwell chamber assay in vitro.Results G31P reduced the proliferation of PC-3 cells in a dose-dependent manner.There was statistically significant difference between 100 ng/mL G31P group at 1st,3rd and 5th day and the control group(P<0.01,P<0.05).PC-3 cells pretreated with 0,1 and 100 ng/mL G31P for 1 day were inoculated on the ECM-coated 96 well plate.The relative adhesion rate was obviously lower(P<0.01).The number of migratory PC-3 cells in 0,1 and 100 ng/mL G31P-treated groups was(35±6),(33±4)and(25±4)respectively.The number of migratory cells in 100 ng/mL G31P-treated group was less than the control group(P<0.05).Conclusion G31P could inhibit proliferation,adhesion and metastasis of the androgen-independent prostate cancer cell line PC-3 in vitro.