反义寡核苷酸对血管瘤内皮细胞VEGF表达的影响 |
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引用本文: | 雷水生,邓镇,朱晓琴,朱璐,张远红,唐晓丹,孙丹,刘光敏.反义寡核苷酸对血管瘤内皮细胞VEGF表达的影响[J].华中科技大学学报(医学版),2012,41(3):341-344. |
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作者姓名: | 雷水生 邓镇 朱晓琴 朱璐 张远红 唐晓丹 孙丹 刘光敏 |
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作者单位: | 1. 广州医学院第五附属医院皮肤科,广州,510700 2. 广州医学院生理学教研室,广州,510700;广州医学院基础学院,广州,510182 3. 广州医学院生理学教研室,广州,510700 |
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摘 要: | 目的探讨反义寡核苷酸(AS-ODN)对培养的血管瘤内皮细胞中血管内皮生长因子(vascular endothelialgrowth factor,VEGF)表达的影响。方法将VEGF反义寡核苷酸(AS-ODN)、正义寡核苷酸(S-ODN)和错义寡核苷酸(M-ODN)分别转染至体外培养的皮肤血管瘤内皮细胞中,采用RT-PCR技术检测各组细胞中VEGF mRNA的表达水平;ELISA法检测各组细胞培养上清液中VEGF蛋白分泌的改变。结果 RT-PCR结果显示:AS-ODN组VEGF mR-NA表达水平较空白对照组明显下降,而S-ODN组、M-ODN组与空白对照组比较,其VEGF mRNA表达未见明显改变。ELISA结果显示:AS-ODN组细胞培养上清液中的VEGF蛋白分泌浓度较空白对照组明显下降,而S-ODN组、M-ODN组与空白对照组比较,VEGF浓度未见明显变化。结论 VEGF AS-ODN转染可以有效地抑制血管瘤内皮细胞VEGF mRNA表达,降低VEGF蛋白分泌,从而有效地抑制血管瘤内皮细胞的生长。
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关 键 词: | 反义寡核苷酸 血管内皮生长因子 蛋白分泌 |
Effect of Antisense Oligonucleotide on Expression of Vascular Endothelial Growth Factor in Cultured Skin Hemangioma Endothelial Cells |
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Institution: | Lei Shuisheng1,Deng Zhen2#,Zhu Xiaoqin2 et al 1Department of Dermatology,the Fifth Affiliated Hospital,2Department of Physiology, Guangzhou Medical College,Guangzhou 510700,China |
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Abstract: | Objective To investigate the impact of antisense oligonucleotides(AS-ODN)on expression of vascular endothelial growth factor(VEGF)in cultured skin hemangioma endothelial cells.Methods AS-ODN,S-ODN and M-ODN were transfected into skin hemangioma endothelial cells respectively.The expression of VEGF mRNA was detected by using RT-PCR.The secretion change of VEGF protein was detected by using ELISA in culture supernatants of skin hemangioma endothelial cells.Results RT-PCR revealed that the expression level of VEGF mRNA in AS-ODN-treated group was significantly decreased as compared with that in blank control group.There was no significant change in the expression of VEGF mRNA in S-ODN-treated group,M-ODN-treated group and control group.ELISA demonstrated that the secretion of VEGF protein in AS-ODN-treated group was significantly decreased as compared with that in control group,but no obvious changes in the secretion of VEGF were found in S-ODN-treated group,M-ODN-treated group and control group.Conclusion VEGF AS-ODN transfection can effectively inhibit the expression of VEGF mRNA in hemangioma vascular endothelial cells and reduce VEGF protein secretion,which can effectively inhibit the growth of hemangioma vascular endothelial cells. |
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Keywords: | antisense oligonucleotide vascular endothelial growth factor protein secretion |
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