抗人转铁蛋白受体单克隆抗体增强姜黄素对雄激素非依赖性前列腺癌的抑制效应

目的检测抗人转铁蛋白受体单克隆抗体(TfR mAb)对雄激素非依赖性前列腺癌细胞生物学行为的影响,了解抗人TfR mAb对姜黄素抗瘤作用的协同效应。方法利用杂交瘤细胞株制备抗人TfR mAb,分别采用CFSE染色、AnnexinⅤ-PI染色和PI染色法流式细胞仪检测抗人TfR mAb对PC-3细胞增殖、凋亡和细胞周期的影响。采用FITC标记的鼠抗人TfR mAb检测姜黄素对雄激素非依赖性前列腺癌细胞表面TfR表达的影响。采用流式细胞仪进一步检测抗人TfR mAb联合姜黄素对PC-3细胞增殖、凋亡和细胞周期的影响。结果抗人TfR mAb对PC-3细胞增殖、凋亡和细胞周期无明显影响(均P>0.05)。姜黄素能够显著上调雄激素非依赖性前列腺癌细胞表面TfR表达(P<0.05)。抗人TfR mAb可显著增强姜黄素对PC-3细胞凋亡的诱导作用(P<0.05),而不影响姜黄素对PC-3细胞增殖和细胞周期的影响(均P>0.05)。结论姜黄素可导致雄激素非依赖性前列腺癌细胞表面TfR表达增高。抗人TfR mAb单独作用对雄激素非依赖性前列腺癌细胞的生物学行为无显著影响,但抗人TfR mAb可以增强姜黄素对雄激素非依赖性前列腺癌细胞的凋亡效应。

An Anti-transferrin Receptor Antibody Enhances the Apoptosis-inducing Effects of Curcumin in Androgen-independent Prostate Cancer Cells

Objective To study the anti-tumor activity of anti-human transferrin receptor monoclonal antibody(TfR mAb)in androgen-independent prostate cancer in vitro and investigate the effect of TfR mAb on the anti-tumor activity of curcumin.Methods After curcumin treatment,transferrin receptor expression was detected by using flow cytometry(FCM)in androgen-independent prostate cancer cells.The viability of prostate cancer cells after TfR mAb treatment was determined by MTT assay.After treatment with TfR mAb and curcumin,the proliferation of prostate cancer cells was assessed by CFSE dilution.FCM was used to analyze the cell cycle and the induction of apoptosis in tumor cells.Results Curcumin could increase the transferrin receptor expression of androgen-independent prostate cancer cells significantly(P<0.05).Anti-human TfR mAb alone had no significant effect on the proliferation,apoptosis and cell cycle of androgen-independent prostate cancer cells(P>0.05).But anti-human TfR mAb could enhance the inhibitory effect of curcumin on the apoptosis of PC-3 cells significantly(P<0.05).Conclusion Curcumin treatment could increase the transferrin receptor expression of androgen-independent prostate cancer cells.Anti-human TfR mAb alone had no obvious anti-tumor activity in androgen-independent prostate cancer cells.Blocking the transferrin receptor could enhance the apoptosis-inducing effect of curcumin on the proliferation of androgen-independent prostate cancer cells.