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Evaluation of Mannitol Salt Agar for Detection of Oxacillin Resistance in Staphylococcus aureus by Disk Diffusion and Agar Screening
Authors:  nter Kampf, Christoph Lecke, Ann-Katrin Cimbal, Klaus Weist,   Henning Rü  den
Affiliation:Günter Kampf, Christoph Lecke, Ann-Katrin Cimbal, Klaus Weist, and Henning Rüden
Abstract:Mannitol salt agar was evaluated for detection of oxacillin resistance in 136 Staphylococcus aureus isolates. All mecA-positive isolates (n = 54) were correctly categorized as oxacillin resistant by the disk diffusion test (1-μg disk; zone diameter, <16 mm); the specificity was 97.6%. Agar screening (2 μg of oxacillin per ml) revealed a sensitivity of 98.1% and a specificity of 95.1%.
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