广州产长春花中文多灵生物碱的年内变化

目的探讨广州长春花中文多灵生物碱在一年内的变化规律,为长春花GAP种植提供依据。方法采用Yilite C18柱(250 mm×4.6 mm,10μm),流动相为甲醇-水(62∶38,二乙胺调pH11),流速1 mL.min-1,检测波长254 nm。结果采用HPLC测定了广州长春花不同样品中文多灵生物碱的含量。结论一年的生长时期中,广州长春花中文多灵生物碱的含量差异较大。茎中文多灵的含量在10～2月处于上升阶段,2～9月总体处于下降阶段。叶中文多灵的含量则在10～3月与4～9月,分别呈现上升与下降的趋势。花与果中文多灵生物碱的含量动态变化与茎、叶相比,较为复杂。茎、叶、花、果中文多灵的含量分别在2月、3月、6月及4月达到最大值。

Study on the vindoline content change in Catharanthus roseus during one year

OBJECTIVE To study the change regularity of vindoline contents in periwinkle Catharanthus roseus Don.of Guangzhou during one year,and provide the basis for the GAP planting.METHODS Yilite C18 column was used with methanol-water(62∶38)(adjusted to pH11 with diethylamine)serving as the mobile phase at flow rate oflmL.min-1,and detected at 254 nm.RESULTS The vindoline contents in periwinkle samples of Guangzhou were successfully determined with the method established.CONCLUSION The vindoline content in periwinkle samples of Guangzhou varied greatly in one year.The vindoline content in stem was raised from October to February and fell from February to September.In leaf,the vindoline content was up from October to March and down from April to September.The vindoline content in flower and fruit were more complex compared to stem and leaf.The maximum vindoline contents in stem,leaf,flower and fruit were in February,March,June and April,respectively.