| 脂多糖对小鼠巨噬细胞TREM-1表达的影响 |
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| 引用本文： | 宋卓慧,孙国瑛,周勇,周慧芳,谢辉,管茶香.脂多糖对小鼠巨噬细胞TREM-1表达的影响[J].长治医学院学报,2012,26(2):81-83. |
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| 作者姓名： | 宋卓慧 孙国瑛 周勇 周慧芳 谢辉 管茶香 |
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| 作者单位： | 1. 中南大学湘雅医学院,410078;长治医学院生理学教研室
2. 中南大学湘雅医学院,410078 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的:初步探讨脂多糖(LPS)应激下小鼠巨噬细胞TREM-1的表达。方法:以小鼠巨噬细胞为研究对象,分别加入不同浓度的LPS(0.04、0.2、1、5和25μg/mL)作用后,采用RT-PCR法检测不同时间点LPS应激的巨噬细胞TREM-1mRNA的表达。结果:LPS呈时间依赖性地上调巨噬细胞TREM-1mRNA的表达,且在LPS作用6h达到峰值;LPS呈剂量依赖性地上调巨噬细胞TREM-1mRNA的表达,且在1μg/mL达到峰值。结论:LPS可上调小鼠巨噬细胞TREM-1mRNA的表达。
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| 关 键 词： | 髓样细胞表达的触发受体-1 急性肺损伤 巨噬细胞 脂多糖 |
Lipopolysaccharide Upregulates the Expression of Triggering Receptor Expressed on Myeloid cells-1 in Murine Macrophage |
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| Institution: | Song Zhuohui,Sun Guoying,Zhou Yong,et al Department of Physiology,Xiangya School of Medicine |
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| Abstract: | Objective:To investigate the effect of lipopolysaccharide(LPS) on the expression of TREM-1 in murine macrophage.Methods:A series of LPS(0.04,0.2,1,5 and 25 μg/mL) were added to the murine macrophage,and the cells were harvested at different time points to obverse the expression of TREM-1 mRNA by RT-PCR.Results:LPS upregulated the expression of TREM-1 mRNA in a time-dependent manner,which reached a peak at 6 h.LPS upregulated the expression of TREM-1 mRNA in a dose-dependent manner,which reached a peak at the concentration of 1 μg/mL.Conclusion:LPS upregulates the expression of TREM-1 in murine macrophage. |
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| Keywords: | Triggering receptor expressed on myeloid cells-1 Acute lung injury Macrophage Lipopolysaccharide |
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