|
|||||
|
|
| 新型氮芥衍生物XHH诱导K562细胞凋亡的研究 | |
| 引用本文： | 苗久旺,张忠泉,张亚宏,李骞,赵瑾,谢松强,王超杰.新型氮芥衍生物XHH诱导K562细胞凋亡的研究[J].华西药学杂志,2012,27(1):5-8. |
| 作者姓名： | 苗久旺 张忠泉 张亚宏 李骞 赵瑾 谢松强 王超杰 |
| 作者单位： | 1. 河南大学化学生物学研究所,河南开封475004;山东中医药高等专科学校药理学教研室,山东烟台264100 2. 河南大学化学生物学研究所,河南开封,475004 3. 河南省天然药物与免疫工程重点实验室,河南开封,475004 4. 河南大学化学生物学研究所,河南开封475004;河南省天然药物与免疫工程重点实验室,河南开封475004 |
| 基金项目： | 国家自然科学基金，国家重大研究计划培育项目，中国博士后科学基金，河南省高等学校青年骨干教师资助计划 |
| 摘 要： | 目的探讨新型氮芥衍生物N-4-(二氯乙基)丁胺]-1,8-萘酰亚胺(XHH)对K562细胞的抗肿瘤作用及其机制。方法采用MTT法检测XHH对K562细胞增殖的影响;用Annexin V-FITC/Hoechst33342、PI/Hoechst33342和Rh123/Hoechst33342双染法高内涵观察细胞的形态学变化、凋亡率及线粒体膜电位;用分光光度法检测caspase-3、caspase-9的活性。结果在一定浓度范围内,XHH能抑制K562细胞的增殖且抗肿瘤活性优于美法仑,呈剂量和时间依赖性地诱导细胞凋亡、降低线粒体膜电位、活化caspase-3和caspase-9。结论 XHH具有较好的抗肿瘤活性,可通过线粒体/caspase-9/caspase-3途径诱导K562细胞凋亡。 |
| 关 键 词： | 氮芥衍生物 抗肿瘤 凋亡 Caspases |
Research on apoptosis of K562 cells induced by a novel nitrogen mustard derivative XHH |
|
| MIAO Jiu-wang , ZHANG Zhong-quan , ZHANG Ya-hong , LI Qian , ZHAO Jin , XIE Song-qiang , WANG Chao-jie.Research on apoptosis of K562 cells induced by a novel nitrogen mustard derivative XHH[J].West China Journal of Pharmaceutical Sciences,2012,27(1):5-8. | |
| Authors: | MIAO Jiu-wang ZHANG Zhong-quan ZHANG Ya-hong LI Qian ZHAO Jin XIE Song-qiang WANG Chao-jie |
| Institution: | 1.Institute of Chemical Biology,Henan University,Kaifeng,Henan,475004 P.R.China;2.Department of Pharmacology,Shandong college of Traditional Chinese Medicine,Yantai,Shandong,264100 P.R.China;3.The Key Laboratory of Natural Medicine and Immuno Engineering,Kaifeng,Henan,475004 P.R.China) |
| Abstract: | OBJECTIVE To investigate the antitumor effects and its possible mechanisms of a novel nitrogen mustard derivative(XHH) on K562 cells.METHODS Cell proliferative effect was assessed by MTT assay.The changes of morphology,apoptotic rate and mitochondrial membrane potential(MMP) were assessed by AnnexinV-FITC/Hoechst33342,PI/Hoecsht33342 and Rh123/Hoechst33342 double staining using high content screening(HCS),respectively.The activation of caspase-3,caspase-9 was evaluated by spectrophotometric method.RESULTS XHH showed a better proliferation inhibition effection on K562 cells than Melphalan due to its induction of apoptosis in a dose-and time-dependent manner,reduction of MMP,and activation of caspase-3 and caspase-9.CONCLUSION XHH showed a nice antitumor activity,induced apoptosis of K562 cells via caspase-9/caspase-3 mitochondrial pathway. |
| Keywords: | Nitrogen mustard derivative Antitumor Apoptosis Caspases |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |