| LC-MS/MS法测定大鼠血浆中的利福布丁 |
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| 引用本文： | 刘晓茜,姚亚敏,马芳,张丽军. LC-MS/MS法测定大鼠血浆中的利福布丁[J]. 华西药学杂志, 2012, 27(2): 184-186 |
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| 作者姓名： | 刘晓茜 姚亚敏 马芳 张丽军 |
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| 作者单位： | 上海市公共卫生临床中心,上海,201508 |
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| 基金项目： | 王宝恩肝纤维化基金，中央高校基本科研业务费专项资金 |
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| 摘 要： | 目的用LC-MS/MS法测定大鼠血浆中利福布丁的浓度。方法以替米沙坦为内标,加入0.2 mL血浆,用1 mL乙酸乙酯萃取,高速离心后上清液氮气吹干并用流动相复溶,取20μL进样检测。结果利福布丁的最低检测限为0.5 ng.mL-1,在0.3～9.6μg.mL-1内线性良好,低、中、高浓度的提取回收率均大于85%,批内、批间RSD低于10%,成功检测了大鼠血浆中利福布丁的血药浓度。结论所用方法灵敏度好、准确度高、分析时间短、样品处理更简便,适用于测定利福布丁的血药浓度。
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| 关 键 词： | 利福布丁 液质色谱联用技术 液液萃取 血药浓度 |
Determination of Rifabutin in rats plasma by LC - MS/MS |
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| LIU Xiao-qian , YAO Ya-min , MA Fang , ZHANG Li-jun. Determination of Rifabutin in rats plasma by LC - MS/MS[J]. West China Journal of Pharmaceutical Sciences, 2012, 27(2): 184-186 |
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| Authors: | LIU Xiao-qian YAO Ya-min MA Fang ZHANG Li-jun |
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| Affiliation: | (Shanghai Public Health Clinical Center,Shanghai,201508 P.R.China) |
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| Abstract: | OBJECTIVE To develop an LC-MS/MS method for determination of Rifabutin in rats plasma.METHODS 0.2 mL plasma samples were mixed with 1 mL ethyl acetate followed by extraction and centrifugation.The supernatant was dried by N2 and the re-dissolied by the mobile phase,20 μL of which was detected by LC-MS/MS.RESULTS The method was linear for Rifabutin in the range of 0.3-9.6 μg·mL-1.The LLOD was 0.5 ng·mL-1.The extraction recoveries of low,middle and high concentration were all higher than 85%.The intra-day and inter-day precisions(RSD) were both less than 10%.CONCLUSION The LC-MS/MS method was simple,sensitive and accurate.It was suitable for the determination of rifabutin in plasma. |
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| Keywords: | Rifabutin LC-MS/MS Liquid-liquid extraction Drug plasma concentration |
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