山芝麻抗鸭体内乙肝病毒及保肝作用的实验研究

目的研究山芝麻水提取物对鸭乙肝病毒(DHBV)的抗病毒与保护肝细胞的作用。方法将DHBV-DNA阳性的麻鸭随机分为高、中、低剂量山芝麻组、拉米夫定组和模型组,分别给予相应药物进行干预,并于用药前、用药第7、14天及停药后第7天取静脉血,检测血清中DHBV-DNA滴度和DHBsAg水平,以及测定丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)和总胆红素(TBiL)的含量。实验结束时,取肝脏组织作病理学检查。结果与模型组比较,山芝麻高剂量组、拉米夫定组用药后血清中DHBV-DNA、DHBsAg、ALT/AST的水平均明显降低(P<0.05或P<0.01),停药后7 d,山芝麻高剂量组的DHBV-DNA、DHBsAg、ALT/AST仍能维持在较低水平(P<0.05),而拉米夫定组则出现明显反跳现象;山芝麻中剂量组显示有一定的效果,小剂量组无明显抑制作用。山芝麻各剂量组血清中TBiL水平没有明显变化(P>0.05)。镜下观察山芝麻高剂量组与阳性药组鸭肝脏的病理改变有一定的改善。结论山芝麻连续用药后有一定的抗炎及护肝作用。

Experimental study of the effect of water extracts from Helicteres angustifolia on anti -hepatitis B virus and protecting hepatocyte activity in vitro

OBJECTIVE To study the effects of the water extracts from Helicteres angustifolia L.(HA) on anti-duck hepatitis B virus(DHBV) and protecting hepatocyte activity.METHODS Ducks with congenital infection of DHBV were randomly divided into five groups:high,medium,low-dose HA treated group,lamivudine-treated group and untreated group.The ducks in the Lamivudine-treated group were fed Lamivudine with a dose of 50 mg·kg-1 once.Ducks in the three-dose HA treated groups were treated with different doses of HA for 14 days respectively.The serum content of DHBV-DNA,DHBsAg,ALT/AST and TBiL were determined before and at the 7 th,14 th day during treatment,and at the 7th day after finishing the treatment.The histopathological changes of liver were evaluated after the treatment.RESULTS Lamivudine showed a rapid inhibiting effect on DHBV-DNA,DHBsAg and ALT/AST,but this effect didn’t last long,and their serum level increased again after treatment.The level of DHBV-DNA,DHBsAg and ALT/AST dropped markedly in the high-dose HA treated group at the 7th and 14th day.Medium-dose HA also showed certain inhibiting effect on them.There was no significant difference in TBiL level among HA groups and control group.The hepatic pathological change in the high-dose HA treated group and positive control group was slighter than that in the model control group.CONCLUSION HA has anti-inflammatory and protecting liver effects.