沙棘原花青素对糖尿病小鼠心肌保护作用机制的研究

目的:初步探讨沙棘原花青素(Sea buckthom procyanidins,SBPC)对糖尿病小鼠心肌组织氧自由基的影响。方法:6月龄ICR小鼠,腹腔注射链脲佐菌素(STZ)150mg/kg制备糖尿病小鼠模型,小鼠随机分为糖尿病心肌病变组(DC)、SBPC高、中、低剂量组和二甲双胍阳性药物对照组(Metformin,MET),每组20只。以正常小鼠为对照,每周测体重和血糖一次,4周后形成早期糖尿病心肌病变,持续4周给药,第5周应用分光光度计法测定各组小鼠心肌组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和总一氧化氮合酶(NOS)活性及丙二醛(MDA)含量。结果:与糖尿病心肌病变组比较,SBPC高、中、低剂量组MDA含量、NOS活性明显降低,GHS-Px和SOD活性明显增强。结论:SBPC对6月龄糖尿病心肌病变小鼠心肌组织氧化损伤有减轻作用。

Protective Effects of Sea Bucithorn Procyanidins on Myocardial Tissue in Diabetic Rats

Objective:To detect the effects of seabuckthom procyanidins on the free radicals in 6-month rats of diabetic cardiomyopathy.Methods:Diabetic rat model was established via intraper-itoneal administration of streptozotocin(STZ).Rats were randomly divided into diabetic cardiomyopathy group(DC),seabuckthom procyanidins groups(large,medium and small dose)(SBPC),metformin group(MET),Using normal rats as the control,body weight and blood glucose were measured weekly.Early diabetic cardiomyopathy was expected in 4 weeks.The whole process of research lasted 4 weeks.Superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),nitrogen monoxidum(NOS)and the content of lipid peroxide malondialdehyde(MDA)in myocardial tissue were detected by spectrometry.Results:Compared with DC group,NOS and the content of MDA in myocardial tissue were significantly reduced while SOD and GSH-Px were increased markedly.Conclusion:Seabuckthom procyanidins ameliorated the damage caused by free radicals in 6-month rats of diabetic cardiomyopathy.