OPG RANKL在2型糖尿病大鼠血清龈沟液中的表达

目的:通过检测2型糖尿病、牙周炎大鼠龈沟液和血清中骨保护素(OPG)和核因子-κB受体活化因子配体(RANKL)的表达水平,探讨OPG和RANKL在2型糖尿病牙周炎发生、发展中所起的作用。方法:通过结扎、喂食高脂高糖、注射链脲佐菌素的方法,分别建立单纯牙周炎和2型糖尿病大鼠实验模型,并通过血糖、胰岛素敏感性指数、牙周情况的检查,确认建模成功。第8周末检测龈沟液和血清中OPG和RANKL的表达水平并进行统计学分析。结果:与对照组相比,牙周炎大鼠龈沟液OPG表达减少,RANKL表达增加(P<0.05);2型糖尿病大鼠龈沟液OPG表达减少,RANKL表达增加(P<0.05);血清中OPG、RANKL表达均增加(P<0.05)。结论:OPG与RANKL的失衡可能是导致牙周炎发生发展的主要原因之一,而作为全身因素的2型糖尿病可能造成宿主本身对牙周炎的高度易感性,加快OPG与RANKL的失衡。

Expressions of OPG and RANKL in Gingival Crevicular Fluid and Blood Serum of Rats with Type 2 Diabetes

Objective:To explore whether osteoprotegerin(OPG) and receptor activator of nuclear factor-κB ligand(RANKL) may play a role in periodontitis and type 2 diabetes pathogenesis through a study of expression level of OPG and RANKL in ingingival crevicular fluid(GCF) and blood serum of type 2 diabetes and periodontitis rat model.Methods:Rats experimental periodontitis and type 2 diabetes model was established respectively by ligation,feeding with the diet enriched with high glucose and high fat and injecting streptozotocin(STZ).By the end of the 8th week,the expression level of OPG and RANKL was detected and compared in ingingival crevicular fluid(GCF) and blood serum each group.Results:The animal models have been successfully established through the examination of blood glucose,insuline sensitivity index and periodontal status.The expression of OPG in GCF of periodontitis group was decreased obviously than those in control group and the expression of RANKL was increased obviously(P<0.05).The expression of OPG in GCF of type 2 diabetic group decreased and the expression of RANKL was increased(P<0.05);The expression of OPG and RANKL in blood serum of type 2 diabetic periodontitis group were increased(P<0.05).Conclusion: The imbanlance of OPG and RANKL may be main reason resulting in periodontitis,Type 2 diabetes as the general factor may cause the host high susceptibility to periodontitis and accelerate the imbalance of OPG and RANKL.