| 5'-核苷酸酶在白血病中的分布特点 |
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| 引用本文： | 赵轼轩,刘津华,董树旭,张华梅,王玫,李庆华,庞天翔,茹永新.5'-核苷酸酶在白血病中的分布特点[J].临床血液学杂志,2010(6). |
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| 作者姓名： | 赵轼轩 刘津华 董树旭 张华梅 王玫 李庆华 庞天翔 茹永新 |
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| 作者单位： | 中国医学科学院中国协和医科大学血液学研究所血液病医院; |
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| 摘 要： | 目的:研究5'-核苷酸酶(5'-NT)在不同类型白血病、骨髓增生异常综合征(MDS)、非白血病患者和正常人中的分布特点。方法:分离100例不同白血病、3例MDS患者、9例非白血病患者和6例正常人骨髓有核细胞,以5'-单磷酸胞嘧啶核苷(5'-CMP)为底物进行细胞组织化学反应,电镜半定量分析5'-NT阳性细胞率和阳性指数。结果:5'-NT阳性反应产物主要位于有核细胞胞膜。急性髓系白血病(AML)M1、M2a、t(8;21)、t(15;17)各组细胞阳性率和阳性指数依次降低,阳性率组间差异有统计学意义(P0.01),阳性指数差异无统计学意义;M1、M2a、t(8;21)、t(15;17)和M5患者5'-NT阳性细胞率和阳性指数均显著高于MDS、非白血病患者和正常人,差异有统计学意义(P0.01);急性B淋巴细胞白血病(B-ALL)患者5'-NT阳性细胞率和阳性指数高于慢性淋巴细胞白血病(CLL),差异有统计学意义(P0.05);CLL、MDS、非白血病患者和正常人的5'-NT阳性细胞率和阳性指数低,组间及两两比较差异无统计学意义。结论:不同白血病患者胞膜5'-NT表达与白血病细胞分化程度负相关。
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| 关 键 词： | 白血病 5'-核苷酸酶 电镜 细胞组织化学 |
Characteristic distribution of 5' Nucleotides in leukemia subtypes |
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| ZHAO Shixuan LIU Jinhua DONG Shuxu ZHANG Huamei WANG Mei LI Qinghua PANG Tianxiang RU Yongxin.Characteristic distribution of 5' Nucleotides in leukemia subtypes[J].Journal of Clinical Hematology,2010(6). |
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| Authors: | ZHAO Shixuan LIU Jinhua DONG Shuxu ZHANG Huamei WANG Mei LI Qinghua PANG Tianxiang RU Yongxin |
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| Institution: | ZHAO Shixuan LIU Jinhua DONG Shuxu ZHANG Huamei WANG Mei LI Qinghua PANG Tianxiang RU Yongxin(Institute of Hematology & Blood Diseases Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Tianjin,300020,China) |
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| Abstract: | Objective:To study distribution of 5'nucleotides(5'-NT) in patients with various leukemia,MDS,non-leukemic diseases,and healthy adults.Method:The nucleated cells were isolated from bone marrow of 100 patients with various leukemia subtypes,3 of MDS,9 of non-leukemic patients and 6 healthy donors.The reactivity of 5'-NT was demonstrated by cytochemistry of 5'-cytidine monophosphate(5'-CMP) .The positive cell ratio and their index were measured with electron microscope on the base of ultrastructural observati... |
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| Keywords: | leukemia 5'nucleotidase electron microscopy cytochemistry |
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