榄香烯抑制表皮生长因子诱导的晶状体上皮细胞增殖的信号转导机制

目的：研究榄香烯（elenene，Ele）抑制重组人表皮生长因子（recombinant human epidermal growth factor，rhEGF）诱导的晶状体上皮细胞（lens epithelium cell，LEC）增殖时对细胞内钙（Ca^2＋）、环磷酸腺苷（cyclic adenosine monophosphate，cAMP）、环磷酸鸟苷（cyclic guanosine monophosphate，cGMP）的影响，探讨其抑制增殖的细胞信号转导机制。方法：采用rhEGF诱导LEC增殖后，用荧光分光光度法检测LEC内钙离子浓度（Ca^2＋]i）、放射免疫分析法检测LEC内cAMP和cGMP含量，探讨Ele的影响。结果：（1）Ele组LEC内Ca^2＋]i；比增殖组显著增高（P〈0．01）；（2）增殖组LEC内cAMP浓度比对照组明显下降、cGMP浓度比对照组明显升高（P〈0．01）；（3）Ele组LEC内cAMP浓度比增殖组显著升高、cGMP浓度比增殖组显著降低（P〈0．01）。结论：Ele抑制LEC增殖的作用是通过细胞内Ca^2＋]i、cAMP和cGMP信号系统及多条信号转导途径的相互作用实现的，这可能是Ele抑制LEC增殖、防治后发性白内障的细胞和分子生物学机制。

Signal transduction mechanism of inhibition of elemene on proliferation induced by recombinant human epidermal growth factor in lens epithelial cell

AIM:To investigate the effects of elemene(Ele) on intracellular concentrations of calcium(i),cyclic adenosine monophosphate(cAMP)and cyclic guanosine monophosphate(cGMP) in lens epithelial cell(LEC) of proliferation induced by recombinant human epidermal growth factor(rhEGF), and the mechanism of signal transduction of inhibiting proliferation.METHODS: Proliferations of LEC were induced by rhEGF;i concentrations of LEC were determined by spectrofluoremeter;The intracellular concentrations of cAMP and cGMP were measured by radioimmunoassay.RESULTS:(1) i of LEC in the Ele group increased obviously compared with the proliferation group(P<0.01);(2)Concentrations of cAMP of LEC in the proliferation group decreased compared with the control group.Concentrations of cGMP of LEC in the proliferation group increased compared with the control group(P<0.01);(3)Concentrations of cAMP of LEC in the Ele group increased significantly compared with the proliferation group.Concentrations of cGMP of LEC in the Ele group decreased compared with the proliferation group(P<0.01).CONCLUSION: Elemene inhibits proliferation of LEC by means of signal transduction of i,cAMP,cGMP and interaction of multiple signal transduction,which may be the cellular and molecular mechanism of Ele inhibiting LEC proliferation and preventing after cataract.