黄芪注射液对人巨细胞病毒感染的粒-单系祖细胞体外增殖的影响

目的：探讨黄芪注射液对人巨细胞病毒(HCMV)感染的粒—单系祖细胞(CFU-GM)体外增殖的影响。方法：采用造血祖细胞体外培养技术，用黄芪干预HCMV感染的CFU-GM，观察计数CFU-GM的产率，大小，峰期及维持时间。结果：①黄芪组每2×105个细胞中CFU-GM簇和集落的产率分别为 333.33±12.69 和81.23±4.93，病毒组每2×105个细胞中簇和集落的产率分别为 167.80±16.63，53.70±1.67，两者相比差异有显著性(P<0.01)。②黄芪组大集落的比例占(9.5±0.8)%，明显高于病毒组(2.7±1.0)%，P<0.01。③黄芪组大集落峰期出现早，集落和大集落维持时间长。结论：在体外黄芪注射液可以促进HCMV感染的CFU-GM增殖。

Effect of Astragalus on the Proliferation of Colony Forming Units Granulocute/Monocyte infected by Human Cytomegalovirus in Vitro

Objective To study the effect of astragalus on the proliferative ability of colony forming units granulocyte/monocyte (CFU GM) infected by human cytomegalovirus (HCMV) in vitro. Methods With the culture technique of CFU GM in vitro, the proliferation of CFU GM infected by HCMV was assayed in a group treated with astragalus and another one without astragalus. Results ①The numbers of CFU GM clusters and colonies in 2×10 5 cells in the astragalus treated group ( 333.33 ± 12.69 ) and ( 81.23 ± 4.93 ), respectively] were significantly greater than those in the un treated group ( 167.80 ± 16.63 ) and ( 53.70 ± 1.67 ), respectively](P< 0.01 ). ②The proportion of the production of big colonies in the astragalus treated group ( 9.5 ± 0.8 )%] was significantly higher than that in the un treated group ( 2.7 ± 1.0 )%] (P< 0.01 ). ③The peak time of big colonies appeared earlier, and the colony and big colony could last longer in the astagalus treated group compared with the un treated group. Conclusions Astagalus may promote the proliferation of CFU GM infected by HCMV.