| 正常和恶性淋巴细胞膜的流动性及其对PHA、ConA刺激的反应性 |
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| 引用本文： | 杨渝珍,郝天玲,钱明,代吾星,皇甫永穆,张志宏.正常和恶性淋巴细胞膜的流动性及其对PHA、ConA刺激的反应性[J].华中科技大学学报(医学版),1989(4). |
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| 作者姓名： | 杨渝珍 郝天玲 钱明 代吾星 皇甫永穆 张志宏 |
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| 作者单位： | 同济医科大学实验医学研究中心分子生物学研究室
(杨渝珍，郝天玲，钱明，代吾星，皇甫永穆)，江汉石油管理局总医院病理室(张志宏) |
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| 摘 要： | 本实验采用疏水性荧光探针DPH(1,6-diphenyl-1,3,5-hexatriene,1,6-二苯基-1,3,5-己三烯)标记小鼠的淋巴细胞膜,根据荧光偏振值的变化比较正常细胞膜和癌变细胞膜流动性及对外界刺激反应性的差别。实验结果表明癌变淋巴细胞膜的流动性明显增高(P<0.001)。用同样浓度的ConA 10mg/L,PHA 40mg/L刺激后,荧光偏振值的变化幅度和方向也不同于正常细胞。本文还对以细胞膜流动性变化作为癌变指标的可能性作了补充说明。
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| 关 键 词： | 荧光偏振 淋巴细胞膜 膜的流动性 Ts淋巴白血病细胞 ConA PHA |
Membrane Fluidity of Normal Lymphocytes and Leukemic Lymphocytes from "615" Mouse with Lymphaemia and Their Response to the Stimulation of PHA and ConA |
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| Yang Yuzhen,Hau Tianling,Qian Min,Dai Wuxing,Huangfu Yongmu.Membrane Fluidity of Normal Lymphocytes and Leukemic Lymphocytes from "615" Mouse with Lymphaemia and Their Response to the Stimulation of PHA and ConA[J].Journal of Huazhong University of Science and Technology(Health Sciences),1989(4). |
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| Authors: | Yang Yuzhen Hau Tianling Qian Min Dai Wuxing Huangfu Yongmu |
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| Institution: | Yang Yuzhen,Hau Tianling,Qian Min,Dai Wuxing,Huangfu Yongmu Department of Medical Molecular Biology,Research Center of Experimental Medicine,Tongji Medical University,WuhanZhang ZhihongDepartment of Pathology,The First Staff and Worker Hospital,Jianhan Administrative Bureau of Petroleum,Hubei |
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| Abstract: | In our experiment,lymphocyte membrane was labelled by DPH fluorescence probe. The rate of rotation of the probe can be measured as the value of fluorescence polarization(PDPH). It will provide us with useful information about the membrane fluidity of lymphocytes. We observed that the F value (unit of lipid fluidity of membrane)of the cancerated lymphocytes is obviously higher than that of normal ones. Furthermore, the F value of cultured leukemic Ts lymphocytes is the highest. In contrast with the normal spleen T-, or mixed lymphocytes, the response of membrane of malignant lymphocytes to the stimulation of ConA or PHA is reflected in the decrease of PDPH value or the increase of F To our surprise, the F of T- lymphocyte from the "615" mouse not injected with tumour cells is also higher than that of the mixed. The question of the membrane fluidity as a diagnostic index is also discussed. |
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| Keywords: | fluorescence polarization membrane of lymphocytes membrane fluidity Ts lymphocytic leukemia ConA PHA |
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