利用荧光定量PCR研究载脂蛋白C Ⅲ基因多态性与冠心病的相关性

目的分析探讨人载脂蛋白CⅢ（ApoCⅢ）基因多态性与脂代谢异常和冠心病的相关性。方法以400名健康献血者和360例冠心病患者为研究对象,经静脉采血后进行血生化检测和提取全血DNA,通过荧光实时定量PCR（FQ—PCR）,同时分析载脂蛋白CⅢ-625和-455位点变异与冠心病、血脂的关系。结果冠心病组载脂蛋白CⅢ-455C纯合子基因型（101例,70．1％）,明显高于对照组（85例,53．1％）,-625del-455c基因型（49．3％）显著高于对照组（41例,25．6％）。单纯高脂血症组和高脂血症并发冠心病组,载脂蛋白CⅢ-455C基因表达比较高。单纯冠心病组的载脂蛋白CⅢ-455c基因表达与正常组间差异无统计学意义。结论载脂蛋白CⅢ-455C基因与冠心病风险呈正相关,-455C纯合子基因型携带者具有冠心病的高风险。载脂蛋白CⅢ-625（del）与-455（C）有协同增加冠心病风险的作用。

Relation between apolipoprotein CIII gene polymorphism and coronary artery disease

OBJECTIVE: To explore the relation between apolipoprotein CIII (ApoCIII) gene polymorphism and coronary artery disease (CAD). METHODS: Peripheral blood samples were collected from 400 healthy blood donors and 360 CAD patients. Total cholesterol (TC), triglycerides (TG), high-density lipoproteins cholesterol (HDL-C), low-density lipoproteins cholesterol (LDL-C), and ApoCIII were detected. Fluorescence quantitative PCR was used to detect the ApoCIII) gene polymorphisms. Genomic DNA was extracted from whole blood samples. We study the relation between variants of ApoCIII-625 and -455 and CAD by FQ-PCR. Then we explore the relation between variants of ApoCIII-625 and -455 and the concentration of lipid. All objects were measured about Total Cholesterol (TC), Triglycerides (TG), high-density lipoproteins cholesterol (HDL-C), low-density lipoproteins cholesterol (LDL-C) and ApoCIII. RESULTS: The ApoCIII-455C homozygote rate of the CAD patients was 70.1%, significantly higher than that of the healthy persons (53.1%). The ApoCIII-625del-455c phenotype rate of the CAD patients was 49.3%, significantly higher than that of the healthy persons. The ApoCIII-455C expression rates of the patients with hyperlipidemia and those with hyperlipidemia combined with CAD were high. There was no significant difference in the expression of ApoCIII-455c between the CAD patients and the normal persons. CONCLUSION: The gene polymorphisms of ApoCIII-455 increase the risk of CAD. ApoCIII-455C gene is positively related to the risk of CAD. The homozygotes of the ApoCIII-455C gene have high risk of CAD. ApoCIII-455C in cooperation with -625 (del) will increase the risk of CAD.