白细胞介素6基因型及血清水平与食管癌的相关性研究

目的了解食管癌患者白细胞介素6（IL-6）基因启动子-572C／G、-634C／G的多态性,探讨IL-6的基因型及其血清水平与食管癌的关系。方法采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法,检测118例食管癌患者和130例健康对照者IL-6基因多态性,同时采用酶联免疫吸附试验检测IL-6的血清水平。结果IL-6-634C／G基因型和等位基因频率在食管癌组和对照组比较,差异有统计学意义（均P〈0．05）;与对照组相比,G等位基因携带者患食管癌的相对风险是C等位基因的1．759倍（OR=1．759,95％可信区间为1．150～2．691）。食管癌组IL-6水平显著高于对照组（16．9ng／L±5．3ng／L vs 4．6ng／L±2．6ng／L,P〈0．01）,携带G等位基因的食管癌患者血清IL-6水平显著高于不携带者（18．8ng／L±6．1ng／L vs 13．2ng／L±6．0ng／L,P〈0．01）。IL-6基因-572C／G多态性在食管癌组和对照组间比较差异元统计学意义（P〉0．05）。结论IL-6基因-634C／G多态性与食管癌之间可能存在相关关系;携带G等位基因的个体可能通过促进IL石的高度表达进而增加了食管癌的发病风险。

Serum level and genotype of interleukin-6 in patients with esophageal cancer

OBJECTIVE: To investigate the polymorphism of the interleukin-6 (IL-6) gene promoter-572C/G and -634C/G in patients with esophageal cancer, and to study the relation between the serum level and genotype of interleukin-6 and esophageal cancer. METHODS: Peripheral blood samples were collected from 118 patients with esophageal cancer and 130 healthy persons as controls. The polymorphism of IL-6 was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The serum level of IL-6 was determined by enzyme-linked immunosorbent assay (ELISA). RESULTS: The frequency of the genotype GG of the IL-6 (-634) site in the cancer group was 12.7%, significantly higher that that of the control group (3.8%, P<0.05). The risk of esophageal cancer of the G allele carriers was 1.759 times that of the C allele carriers (OR=1.759, 95% CI=1.150-2.691). The serum level of IL-6 of the esophageal cancer group was (16.9+/-5.3) ng/L, significantly higher than that of the control group (4.6+/-2.6) ng/L, P<0.01]. The serum level of IL-6 of the esophageal cancer with the G allele carriers was (18.8+/-6.1) ng/L, significantly higher than that of the esophageal cancer without the G allele carriers (13.2+/-6.0) ng/L, P<0.01]. There was no significant difference in the distribution of the IL-6 gene-572C/G polymorphism between the 2 groups (P>0.05). CONCLUSION: IL-6 gene-634C/G polymorphism is associated with the esophageal cancer. The IL-6 allele G carriers may be at increased risk of the esophageal cancer because of the increase of the IL-6 expression.