补肾通络方治疗去势大鼠骨质疏松的研究

目的:观察补肾通络方对骨质疏松大鼠骨代谢和骨密度的干预效应,并探讨其作用机制。方法:通过卵巢去势的方法造成大鼠骨质疏松模型,造模后随机分为6组:假手术组、模型组、仙灵骨葆组、补肾基础组、通络组、补肾通络组。治疗10周后,检测大鼠体重、血清钙(S-Ca)、血清磷(S-P),血清碱性磷酸酶(S-ALP)及骨密度。结果:与假手术组比较,骨质疏松大鼠体重增加,S-Ca降低,S-P上升,S-ALP上升,骨密度下降,各组均有显著性差异(P0.05)。与模型组相比,各治疗组骨质疏松大鼠体重增长受到抑制,但差异不显著;S-Ca上升,S-P下降,其中补肾通络组有显著差异(P0.05);S-ALP下降,各组均有显著性差异(P0.01);骨密度均上升,补肾通络组骨密度值高于补肾基础组或通络组,其中补肾基础组有显著差异(P0.05),而通络组及补肾通络组有显著差异(P0.01)。结论:补肾通络中药可以有效降低骨质疏松大鼠的骨代谢异常旺盛水平,明显提高其骨密度,补肾中药与通络中药在治疗骨质疏松方面具有良好的协同增效作用。

Empirical Studies on Treatment Effects of Bushen Tongluo Decoction in Osteoporosis Rats

ObJective: To observe the intervention effects and mechanism of Bushen Tongluo decoction on bone metabolism and density in the osteoporosis rats. Method :Rat models of the osteoporosis were established by bilateral ovariotomy. The rats were divided into six groups randomLy:the nomal group,model group,XinLinGuBao group,Bushen-based decoction group, Tongluo decoction group, Bushen Tongluo decoction group. After ten weeks treatment,the rats weight,the serum calcium,the serum phosphorus,the serum alkaline phosphatase and the bone density. Result :The weight of the osteoporosis rats increased, the serum calcium decreased, the serum phosphorus increased, the serum alkaline phosphatase increased and the bone density decreased in model group as compared with the normal group(P<0.05).The weight gain of the osteoporosis rats were inhibited in each treat group as compared with model group,but there were no significant difference. The serum calcium increased, the serum phosphorus decreased in each treat group as compared with model group,and there were significant difference in Bushen Tongluo decoction group.The serum alkaline phosphatase decreased obviously in each group as compared with model group(P<0.01).The bone density increased in each group as compared with model group,and there were significant difference in Bushen-based decoction group.(P<0.05) and in Tongluo decoction group and Bushen Tongluo decoction group(P<0.01). The numerus of Bushen Tongluo decoction group were higher than the numerus of Bushen-based decoction group or Tongluo decoction group. Conclusion :Bushen Tongluo decoction group can effectively decrease high dysbolism of bone metabolism,increase bone density in the osteoporosis rats. Bushen decoction and Tongluo decoction have good synergism.