| 真核绿色荧光蛋白表达载体pEGFP-C3-B7-2的构建 |
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| 引用本文： | 路静,王明臣,赵军,宋谦,崔自由,赵继敏,杨洪艳,黄幼田,汤黎明,赵国强,董子明.真核绿色荧光蛋白表达载体pEGFP-C3-B7-2的构建[J].郑州大学学报(医学版),2005,40(4):583-585. |
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| 作者姓名： | 路静 王明臣 赵军 宋谦 崔自由 赵继敏 杨洪艳 黄幼田 汤黎明 赵国强 董子明 |
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| 作者单位： | 郑州大学基础医学院病理生理学教研室,郑州,450052;郑州大学基础医学院生物化学教研室,郑州,450052;郑州大学基础医学院微生物学与免疫学教研室,郑州,450052 |
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| 基金项目： | 河南省科技攻关基金资助项目0324410034，河南省科技创新人才工程项目20008402 |
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| 摘 要： | 目的：构建含人B7-2基因的绿色荧光蛋白表达载体pEGFP-C3-B7-2。方法：应用RT-PCR、巢式PCR方法从重症肌无力患者胸腺组织中扩增出人B7—2 cDNA基闪片段，经回收、纯化、酶切后，依次连接到质粒pGEM—T和pEGFP—C3上．结果与结论：酶切及测序证实成功构建了克隆载休pGEM—B7—2和真核绿色荧光表达载体pEGFP—C3—B7—2。
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| 关 键 词： | 人B7分子 绿色荧光蛋白 真核表达载体 克隆 |
| 修稿时间： | 2005年3月10日 |
Construction of eukaryotic green fluorescent protein expression vector pEGFP-C3-B7-2 |
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| LU Jing,WANG Mingchen,ZHAO Jun,SONG Qian,CUI Ziyou,Zhao Jimin,Yang Hongyan,Huang Youtian,TANG Liming,ZHAO Guoqiang,DONG Ziming.Construction of eukaryotic green fluorescent protein expression vector pEGFP-C3-B7-2[J].Journal of Zhengzhou University: Med Sci,2005,40(4):583-585. |
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| Authors: | LU Jing WANG Mingchen ZHAO Jun SONG Qian CUI Ziyou Zhao Jimin Yang Hongyan Huang Youtian TANG Liming ZHAO Guoqiang DONG Ziming |
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| Institution: | LU Jing 1),WANG Mingchen 2),ZHAO Jun 1),SONG Qian 1),CUI Ziyou 1),ZHAO Jimin 1),YANG Hongyan 1),HUANG Youtian 1),TANG Liming 1),ZHAO Guoqiang 3),DONG Ziming 1) 1)Department of Pathophysiology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450052 2)Department of Biochemistry,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450052 3)Department of Microbiology and Immunology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450052 |
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| Abstract: | Aim: To construct eukaryotic fluorescent expression vector pEGFP GAAB2 C3 GAAB2 B7 GAAB2 2. Methods: Human B7 GAAB2 2 cDNA in the thymus tissue of patients with myasthenia gravis was amplified using RT GAAB2 PCR and nested PCR. The amplified product was examined by electrophoresis and sequence determination. This fragment was inserted into pGEM GAAB2 T plasmid and pEGFP GAAB2 C3 fluorescent expression vector. Results and Conclusion: The restriction enzyme digestion and the sequence analysis showed that recombined cloning vector pGEM GAAB2 B7 GAAB2 2,and expression vector pEGFP GAAB2 C3 GAAB2 B7 GAAB2 2 had been successfully constructed. |
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| Keywords: | human B7 molecule green fluorescent protein eukaryotic expression vector clone |
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