慢性丙型肝炎患者（2＇，5＇）寡腺苷酸合成酶的活性测定

目的：了解慢性丙型肝炎患者（２＇，５＇）寡腺苷酸合成酶（２－５ＡＳ）活性在干扰素治疗过程中的动态变化及意义。方法：ＰＥＩ－ＣＥＬＬＵＬＯＳＥ薄板层析法。结果：（１）慢性丙型肝炎患者２－５ＡＳ基础水平比正常人显著升高（Ｐ＜０．０１）；（２）ＩＦＮα治疗结束后ＨＣＶＲＮＡ持续阳性的病人２－５ＡＳ基础水平比ＨＣＶＲＮＡ转阴的病人要高（Ｐ＜０．０５）；（３）ＩＦＮα治疗效果较好，ＨＣＶＲＮＡ转阴的病人２－５ＡＳ水平在用药后２４ｈ比ＨＣＶＲＮＡ未转阴的病人上升得更高（Ｐ＜０．０１）；（４）用药后１个月２～５ＡＳ水平与ＩＦＮα治疗效果无显著相关性（Ｐ＞０．０５）。结论：２－５ＡＳ可以作为评价干扰素治疗效果的１个机体反应指标。

2''''-5''''OLIGOADENYLATE SYNTHETASE ACTIVITY IN INTERFERON THERAPY FOR CHRONIC HEPATITIS C

bjective:To determine whether the(2'-5')oligoadenylate synthetase(2-5AS)activity of chronic hepatitis C patient was related to the curative effect of interferon(IFN)therapy. Methods:PEI-CELLULOSE thin-layer chromatography.Results:(1)The 2-5AS activities in PBMC of patients with chronlc hepatitis C before injection were higher than those of normal people(P<0.01);(2)The 2-5AS basal activities in patients whose HCV-RNA disappeared detectable were higher than those in pa-tients whose HCV-RNA disappeared(P<0.05);3)The 2-5AS activities of 24 h after injection in pa-tients with effective treatment(HCV RNA disappeared)increased significantly higher than those in pa-tients with ineffective treatnient(HCV RNAremained detectable,P<0.01);(4)One month after iniec-tion,the activities of 2-5AS in the patients had no significant relation to IFN treatment(P>0.05).Concldsion:2-5AS could be determined as a biological response parameter during the therapy with IFN.