| HPLC色谱法测定贵州黔北凹叶厚朴中和厚朴酚的含量 |
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| 引用本文： | 冯华,王样培,申桂霞,邹孔强,王小东. HPLC色谱法测定贵州黔北凹叶厚朴中和厚朴酚的含量[J]. 现代中药研究与实践, 2011, 0(2): 68-70 |
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| 作者姓名： | 冯华 王样培 申桂霞 邹孔强 王小东 |
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| 作者单位： | 遵义市药品检验所;贵州贵阳中医学院; |
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| 基金项目： | 遵义市中药现代化科技产业研究开发专项项目课题(项目编号:市科合中药专字〔2008〕2号) |
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| 摘 要： | 目的 建立高效液相色谱法测定贵州黔北凹叶厚朴中和厚朴酚的含量.方法 色谱条件:色谱柱为Diamonsil C<,18>以甲醇-水(78:22)为流动相;流速为1.0mL/min;柱温25℃;检测波长为294nm.结果 和厚朴酚线性范围为0.278 4 μg～0.649 6 μg,r=0.999 5;本品的平均回收率为1...
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| 关 键 词： | 高效液相色谱法 黔北凹叶厚朴 和厚朴酚 含量测定 |
Content Determination of Honokiol in QianBeis Magnolia officinalis by HPLC |
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| FENG Hua,WANG Xiang-pei,SHEN Gui-xia,ZOU Kong-qiang,WANG Xiao-dong. Content Determination of Honokiol in QianBeis Magnolia officinalis by HPLC[J]. Research and Practice on Chinese Medicines, 2011, 0(2): 68-70 |
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| Authors: | FENG Hua WANG Xiang-pei SHEN Gui-xia ZOU Kong-qiang WANG Xiao-dong |
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| Affiliation: | FENG Hua1,WANG Xiang-pei2,SHEN Gui-xia1,ZOU Kong-qiang1,WANG Xiao-dong1(1.Zunyi Institute for Drug Control,Zunyi 563002,China,2.Guiyang College of Traditional Chinese Medicine,Guiyang 550002,China) |
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| Abstract: | Objective To establish a method for the content determination of honokiol in QianBeis Magnolia officinalis by HPLC.Methods A Diamonsil C18 column was used with mobile phase of methanol-water（78 ： 22）,the detection wavelength was 294nm,the flow rate was 1.0 mL/min.Results The calibration curve showed a good linearity with the range of 0.627 2~1.164 8 μg,r=0.999 4.The average recovery was 102.12% and RSD was 1.3%.Conclusion The method is simple,sensitive and accurate. |
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| Keywords: | HPLC QianBeis Magnolia officinalis honokiol determination |
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