| 贵州野生与栽培天麻种质资源的SSR分析 |
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| 引用本文： | 陈琦,刘巍,程纪伦.贵州野生与栽培天麻种质资源的SSR分析[J].现代中药研究与实践,2011(2):26-28. |
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| 作者姓名： | 陈琦 刘巍 程纪伦 |
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| 作者单位： | 贵州省人民医院;贵州省血液中心;广东医学院药学院; |
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| 基金项目： | 国家科技攻关项目(2004BA721A33); 贵州省中药专项(黔科中药专字[2003]51号) |
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| 摘 要： | 目的 对贵州省野生和栽培共24个天麻基因组DNA的遗传多态性进行分析,构建其SSR指纹图谱.方法 采用改良的CTAB法提取天麻基因组DNA,采用NTSYSpc软件进行UPGMA聚类分析和遗传相似度分析.结果 改良的CTAB法能够获得纯度和浓度较高的天麻基因组DNA,PCR扩增获得较清晰的SSR指纹图谱,UPGMA聚类可...
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| 关 键 词： | 天麻 SSR 种质资源 |
SSR Analysis on the Germplasm Resources of Gastrodia elata Blume. in Guizhou |
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| CHEN Qi,LIU Wei,CHENG Ji-lun.SSR Analysis on the Germplasm Resources of Gastrodia elata Blume. in Guizhou[J].Research and Practice on Chinese Medicines,2011(2):26-28. |
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| Authors: | CHEN Qi LIU Wei CHENG Ji-lun |
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| Institution: | CHEN Qi1,LIU Wei2,CHENG Ji-lun3(1.Guizhou Provincial Hospital,Guiyang 550002,China,2.Guizhou Provincial Blood Center,3.Pharmacy College,Guangdong Medical University,Dongguan 523808,China) |
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| Abstract: | Objective Get genomic DNA from Genetic Gastrodia elata Blume.for PCR,analyzed genetic polymorphisms of genomic DNA from 24 samples of wild and cultivated Gastrodia elata Blume.in Guizhou,set up the SSR fingerprintings.Methods DNA was obtained by CTAB methods,and 10 primers from Genebank were selected for SSR-PCR,UPGMA dendrogram was analyzed by NTSYSpc with SSR fingerprintings.Results Genetic DNA obtained by CTAB mehods with high purity and cencetration was fit for PCR,got 3 primers from 10 and clear finger... |
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| Keywords: | Gastrodia elata Blume SSR genetic polymorphism |
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