| 附子理中丸中甘草酸含量的HPLC测定 |
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| 引用本文： | 姜秀晶,贾诚,李锦. 附子理中丸中甘草酸含量的HPLC测定[J]. 现代中药研究与实践, 2011, 0(2): 66-68 |
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| 作者姓名： | 姜秀晶 贾诚 李锦 |
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| 作者单位： | 天津中医药大学中药学院;天津天士力之骄药业有限公司; |
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| 摘 要： | 目的 建立附子理中丸中甘草酸的高效液相色谱分析方法.方法 Waters Symmetry C<,18>(4.6mm×250 mm,5 μm),流动相为乙腈-磷酸溶液(0.017 mol/L,40:60),流速1.0 mL·mm<'-1>,检测波长250nm,柱温35℃.结果 甘草酸在30.9～154.4 μg·mL<...
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| 关 键 词： | 附子理中丸 甘草酸 HPLC |
Determination of Glycyrrhizic Acid in Fuzi Lizhong Pills by HPLC |
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| JIANG Xiu-jing,JIA Cheng,LI Jin. Determination of Glycyrrhizic Acid in Fuzi Lizhong Pills by HPLC[J]. Research and Practice on Chinese Medicines, 2011, 0(2): 66-68 |
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| Authors: | JIANG Xiu-jing JIA Cheng LI Jin |
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| Affiliation: | JIANG Xiu-jing1,JIA Cheng1,2,LI Jin1(1.Tianjin University of Traditional Chinese Medicine,Tianjin 300193,China,2.Tianjin Tasly Pride Pharmaceutical Company Limited,Tianjin 300402,China) |
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| Abstract: | Objective To develop an HPLC method for the quantitative determination of glyeyrrhizic acid in Fuzi Lizhong pills.Methods The analysis was carried out on a Waters Symmetry C18（4.6 mm×250 mm,5 μm） column with the mixture of methyl cyanides-phosphoric acid（0.017 mol/L,40 ： 60） as the mobile phase at a flow rate of 1.0 mL·min-1,the detection wavelength was set at 250 nm and the column temperature was maintained at 35 ℃.Results The calibration curve showed a good linearity with the range of 30.9~154.4 μg·mL-1 for glyeyrrhizie acid,and the mean recovery（n=6） was 98.63%（RSD=0.99%）.Conclusion This method is simple and accurate.And it can be used for the determination of glycyrrhizic acid in Fuzi Lizhong pills. |
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| Keywords: | Fuzi Lizhong pills glyeyrrhizic acid HPLC |
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