Amelioration of altered antioxidant enzymes activity and glomerulosclerosis by coenzyme Q10 in alloxan-induced diabetic rats |
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| Authors: | Hassan Ahmadvand Majid Tavafi Ali Khosrowbeygi |
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| Institution: | 1. Department of Pharmacology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;2. Department of Pharmacology and Toxicology, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran;3. Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran;1. Department of Pharmacology, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran;2. Toxicology and Diseases Group, The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Science, Tehran, Iran;3. Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran;4. Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran;5. Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran;6. Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran;7. Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran;8. Nervous System Stem Cells Research Center, Semnan University of Medical Sciences, Semnan, Iran;9. Department of Anatomy, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran;10. Razi Drug Research Center, Iran University of Medical Sciences, Tehran, Iran |
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| Abstract: | Coenzyme Q10 is a natural antioxidant and scavenging free radicals. In the present study, we examined antioxidative activities of coenzyme Q10 and possible protective effect of coenzyme Q10 on in vivo and in vitro lipid peroxidation, antioxidant enzymes activity and glomerulosclerosis in alloxan-induced type 1 diabetic rats. Thirty Sprague–Dawley male rats were divided into three groups randomly: group 1 as control, group 2 as diabetic untreatment, and group 3 as treatments with coenzyme Q10 by 15 mg/kg i.p. daily, respectively. Diabetes was induced in the second and third groups by alloxan injection subcutaneously. After 8 weeks, animals were anaesthetized, liver and kidney were then removed immediately and used fresh or kept frozen until their lipid peroxidation analysis. Blood samples were also collected before killing to measure the lipid peroxidation and antioxidant enzymes activity. Kidney paraffin sections were prepared and stained by periodic acid-Schiff method. Glomerular volume and leukocyte infiltration were estimated by stereological rules and glomerular sclerosis was studied semi-quantitatively. Coenzyme Q10 significantly inhibited leukocyte infiltration, glomerulosclerosis and the levels of malondialdehyde (MDA) serum and kidney content in treated group compared with the diabetic untreated group. Coenzyme Q10 significantly inhibited LDL oxidation in vitro. Coenzyme Q10 significantly increased the serum levels of glutathione (GSH) and serum activity of catalase (CAT) and superoxide dismutase (SOD) in treated group compared with the diabetic untreated group. Coenzyme Q10 alleviates leukocyte infiltration and glomerulosclerosis and exerts beneficial effects on the lipid peroxidation and antioxidant enzymes activity in alloxan-induced type 1 diabetic rats. |
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