| 30 and 32 kDa outer membrane proteins of Bordetella pertussis as a modulator on promoting degranulation of mast cells |
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| 作者姓名： | YONG LIANG LOU~ JIE YAN~ YI HUI LUO~ and YA FEI MAO~~ |
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| 作者单位： | YONG LIANG LOU~1，JIE YAN~2，YI HUI LUO~2 and YA FEI MAO~2~1 Department of Laboratory Medicine，Wenzhou Medical College，82 College Road，Wenzhou，P. R. China~2 Department of Medical Microbiology and Parasitology，College of Medical Science，Zhejiang University，353 Yan-an Road，Hangzhou，P. R. China |
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| 摘 要： | Asthma is a common allergic disease of respiratorytract in human,especiallyin children 1-3] ,andcan be classified into 3 clinical types ,immediateasthmatic reaction (IAR) ,late asthmatic reaction(LAR) and biphasic asthmatic reaction(BAR)4] ,in which approximately 60 %of cases intheworld belong to IgE-mediated IAR5-7] . By theprevious epidemiological studies ,it was reportedthat respiratory infections by some pathogenic mi-croorganisms, such asBordetella pertussis, in-fluenza virus A o…
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| 关 键 词： | 膜蛋白 百日咳 调节器 细胞脱粒 |
30 and 32 kDa outer membrane proteins of Bordetella pertussis as a modulator on promoting degranulation of mast cells |
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| YONG LIANG LOU,JIE YAN,YI HUI LUO and YA FEI MAO.30 and 32 kDa outer membrane proteins of Bordetella pertussis as a modulator on promoting degranulation of mast cells[J].Chinese Journal of Microbiology and Immunology,2005,3(2):111-119. |
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| Authors: | YONG LIANG LOU JIE YAN YI HUI LUO YA FEI MAO |
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| Institution: | [1]Department of Laboratory Medicine, Wenzhou Medical College, 82 College Road, Wenzhou, P. R.China [2]Department of Medical Microbiology and Parasitology, College of Medical Science, Zhejiang University, 353 Yah-an Road, Hangzhou, P. R. China |
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| Abstract: | The correlation between the activities of the outer membrane proteins (OMPs) of Bordetella pertussis and the IgE-mediated asthma was investigated in the present study, in which the OMPs of B.pertussis and their components were prepared by detergent treatment and chromatography, and the molecular weights of the OMPs components were determined by SDS-PAGE. The amounts of total as well as the ovalbumin (OVA)-specific IgE induced by dead B.pertussis whole bacterial vaccine on guinea pigs were detected by ELISA. Meanwhile, the effect of the OMPs and their components to promote the degranulation of guinea pig mast cells was observed by using the mast cell degranulation test, and ELISA assay was used to measure the histamine levels in the supernatants from the mast cell cultures. Histamine sensitive test was used to demonstrate the effects of the OMPs and their components to increase the histamine lethal sensitivity in mice. It was found that four components with molecular weights of 30, 32, 38 and 69 kDa could be obtained from the OMPs of B.pertussis, and the dead whole bacteria vaccine of B.pertussis had the ability to increase the levels of the total as well as the OVA-specific IgE in sera of guinea pigs. The OMPs and their 30 and 32 kDa components demonstrated significantly enhancing effect on the degranulation of guinea pig mast cells, and the histamine levels in the supernatants from the mast cell culture treated with OMPs and their 30 and 32 kDa components were also significantly increased. It is evident that the strong adjuvant activity and the enhancing effect to degranulation of mast cells and the release of histamine of certain outer membrane components of B.pertussis could be demonstrated as revealed by the results of the present study, suggesting the possibility of a close relationship between the infection of vaccination with B.pertussis and the IgE-mediated asthma. |
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| Keywords: | Bordetella pertussis Bacterial outer membrane protein Immunoglobulin E Mast cellsCell degranulation Histamine Asthma |
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