化学计量学方法应用于贝母化学特征指纹图谱的研究

采用高效液相色谱法,以XDP-C18(4.6×250 mm)为色谱柱,甲醇-水体积比为70∶30,内含7.5 mmol/L十二烷基硫酸钠(SDS),pH为4.5±0.1]为流动相,检测波长为215 nm,测定了11种贝母样品,根据样品中不同化学组分的保留时间、相对峰面积,应用化学计量学方法计算出描述相邻两个色谱峰分离程度和质量的重要参数并进行聚类。结果表明,聚类分析的结果与其植物学分类相一致。该种方法可用于贝母的鉴别,并有助于贝母化学特征指纹图谱的建立。

Study on the Application of Chemometrics Methods in Chemical Characteristic Fingerprinting of Fritillaria

A method for determination of eleven samples in Fritillaria by HPLC with UV detection at 215 nm was described. The column was used a XDP-C18, 4.6 x 250 mm, 5 microm, and the mobile phase was methanol-water (volume: volume = 70: 30, to be added 7.5 mmol/L SDS, pH 4.5 +/- 0.1). Important parameters with chromatograms of eleven samples were obtained and crusted. The results indicated that it is similar between clustering analysis and taxonomy of Fritillaria. Identification of Fritillaria and establishment of the chemical characteristic fingerprinting can used the methods.