| CTLA4-Ig融合蛋白诱导猕猴同种异体肝移植免疫耐受的实验研究 |
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| 引用本文： | 胡明道,赵恋峰,于恒海,唐继红,黄明,刘峰,周玉凯,李立.CTLA4-Ig融合蛋白诱导猕猴同种异体肝移植免疫耐受的实验研究[J].中国普外基础与临床杂志,2010,17(1):40-45. |
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| 作者姓名： | 胡明道 赵恋峰 于恒海 唐继红 黄明 刘峰 周玉凯 李立 |
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| 作者单位： | 1. 昆明医学院第二附属医院肝胆外科,昆明,650101
2. 昆明市第一人民医院暨昆明医学院附属甘美医院肝移植中心,昆明,650011 |
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| 摘 要： | 目的探讨细胞毒性T淋巴细胞相关抗原4(CTLA4-Ig)融合蛋白对同种异体肝脏移植免疫耐受的诱导作用及机理。方法利用大型哺乳动物猕猴作为研究对象,建立同种异体原位肝脏移植模型。同种异体原位肝脏移植对照组及CTLA4-Ig组各5只。观察术后生存时间,检测肝功能、IL-2、IL-10、排斥反应的病理学分级和术后肝脏细胞凋亡指数。结果对照组平均存活时间为6.57 d,CTLA4-Ig组平均存活时间为14.92 d(P0.05)。肝功能变化:对照组术后ALT明显升高,Alb则明显降低;CTLA4-Ig组ALT及Alb均维持于正常稳定水平。细胞因子变化:术后3 d起,对照组循环血中IL-2表达水平相对较高,而CTLA4-Ig组IL-10的表达水平较对照组高。移植物病理排斥反应分级:在移植术后CTLA4-Ig组排斥反应程度明显比对照组轻。对照组术后3 d起肝脏细胞凋亡指数较CTLA4-Ig组明显升高。结论CTLA4-Ig融合蛋白可诱导移植后免疫耐受,延长受体生存时间。细胞因子IL-2或者IL-10可作为监测移植排斥反应或者免疫耐受的实验室指标之一。
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| 关 键 词： | 细胞毒性T淋巴细胞相关抗原4 猕猴 肝移植 免疫耐受 |
Cytotoxic T Lymphocyte-Associated Antigen 4 Fusion Protein Induces Immunotolerance of Orthotopic Liver Transplantation in Rhesus Monkeys |
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| HU Ming-dao,ZHAO Lian-feng,YU Heng-hai,TANG Ji-hong,HUANG Ming,LIU Feng,ZHOU Yu-kai,LI Li.Cytotoxic T Lymphocyte-Associated Antigen 4 Fusion Protein Induces Immunotolerance of Orthotopic Liver Transplantation in Rhesus Monkeys[J].Chinese Journal of Bases and Clinics In General Surgery,2010,17(1):40-45. |
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| Authors: | HU Ming-dao ZHAO Lian-feng YU Heng-hai TANG Ji-hong HUANG Ming LIU Feng ZHOU Yu-kai LI Li |
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| Institution: | HU Ming-dao1,ZHAO Lian-feng1,YU Heng-hai1,TANG Ji-hong1,HUANG Ming1,LIU Feng1,ZHOU Yu-kai1,LI Li2.1.Department of Hepatobiliary Surgery,The Second Affiliated Hospital of Kunming Medical College,Kunming 650101,China,2.Liver Transplantation Center,The First People\'s Hospital of Kunming City , Affiliated Ganmei Hospital of Kunming Medical College,Kunming 650011 |
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| Abstract: | Objective To explore the effect of cytotoxic T lymphocyte-associated antigen 4(CTLA4-Ig) fusion protein on the function of orthotopic liver allograft.Methods Orthotopic liver allograft models of rhesus monkeys were established in this study.The survival time,liver function and morphologic changes of graft were observed,respectively.The levels of IL-2 and IL-10 were detected by ELISA.Apoptosis was monitored by TUNEL.Results The average survival of control group was 6.57 d,while the average survival of CTLA4-... |
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| Keywords: | Cytotoxic T lymphocyte-associated antigen 4 Rhesus monkey Liver transplantation Immunotolerance |
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