Hedgehog信号通路在非霍奇金淋巴瘤中的表达及临床意义

Hedgehog(Hh)信号通路在肿瘤的发生和发展过程中发挥重要调控作用。本研究探讨Hh信号通路中Gli1和Gli2在非霍奇金淋巴瘤(non-Hodgkin’s lymphoma,NHL)中的表达及意义。分别采用实时定量RT-PCR和免疫组织化学的方法分析检测18例NHL和10例淋巴结反应性增生组织中Gli1、Gli2 mRNA和蛋白表达水平。结果表明,NHL组织中Gli1和Gli2 mRNA中位表达水平经β-actin校正后分别为2.05和2.31,明显高于淋巴结反应性增生组织(分别为0.82和0.89),且Gli1与Gli2 mRNA表达水平呈正相关(r=0.63,p<0.01),Gli2 mRNA水平与NHL临床分期(p=0.03)具有相关性;Gli1和Gli2蛋白在NHL中的阳性率分别为80%和68%,明显高于对照组,且Gli1和Gli2蛋白表达呈正相关(r=0.62,p<0.05);Gli1和Gli2蛋白表达均与NHL分期相关(p=0.05,p=0.01),而Gli1和Gli2基因及蛋白表达水平与NHL患者年龄比例、性别比例、B症状及分型方面无明显相关性(p>0.05)。结论:Hh信号通路中Gli1和Gli2在NHL患者组织中高表达,并对NHL患者临床分期及预后具有指导意义。进一步对Hh信号通路在NHL中作用机制的深入探讨将有助于揭示NHL的发生、发展的机理,并有望成为NHL未来治疗的重要突破点。

Expression and clinical significances of hedgehog signaling pathway in non-Hodgkin's lymphoma

This study was aimed to investigate the expression and clinicopathologic significance of Gli1 and Gli2,2 factors of Hedgehog（Hh） signaling pathway,in non-Hodgkin′s lymphoma（NHL）.Gli1 and Gli2 mRNA and protein in 18 cases of NHL and 10 cases of reactive lymphadenitis were amplified and identified by real-time PCR,and were assayed by immunohistochemical staining respectivly.The results showed that（1） Gli1 and Gli2 mRNA in NHL group（RQ 2.05,2.31） were expressed higher than that in reactive lymphadenitis group（RQ 0.82,0.89）.Gli1 mRNA activated level was positively related with Gli2（r=0.63,p0.01）.In addition,Gli2 also positively correlated to clinical stages of NHL（p=0.03）,but the expressions of Gli1 and Gli2 mRNA had no significant correlation to B symptoms,blood β2-microglobulin,age and sex.（2） The positive expression rate of Gli1 and Gli2 protein in NHL group were 80% and 68% respectively,which were extremely higher than that in reactive lymphadenitis group.Gli1 protein level was positively related with Gli2（r=0.62,p0.05）.Both Gli1 and Gli2 protein expression positively correlated to clinical staging of NHL（p=0.05,p=0.01）.It is concluded that the Gli1 and Gli2 of Hh signaling pathway have been found to higher express in patients with NHL,and have significance for clinical staging and predicting prognosis of NHL.To further investigate the role of Hh signaling pathway in NHL will contribute to elucidate the occurence and development of NHL,and provide a favorable method for therapy of NHL.