| 间期荧光原位杂交和常规染色体分析诊断急性早幼粒细胞白血病的比较 |
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| 作者姓名： | Wang R Miao KR Qiu HR Qian SX Hong M Qiao C Zhag JF Fan L Wu HX Lu H Qiu HX Chen LJ Zhag SJ Xu W Liu P Li JY |
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| 作者单位： | 南京医科大学第一附属医院、江苏省人民医院血液科,江苏,南京,210029 |
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| 基金项目： | 南京K科大学第一附属创新团队工程资助,江苏省高校优势学科建设工程资助项目 |
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| 摘 要： | 本研究主要探讨急性早幼粒细胞白血病(APL)细胞遗传学特征并比较间期荧光原位杂交技术(I-FISH)和常规染色体核型分析技术(CC)。应用常规染色体核型分析及I-FISH技术对157例APL患者细胞遗传学特征进行研究,采用短期培养法制备骨髓细胞染色体,应用染色体R显带技术对136例拟诊APL患者进行常规细胞遗传学检测,其中对45例同时进行了CC和I-FISH检测,对其余21例仅进行I-FISH分析。结果表明:136例进行CC分析的APL患者中,120例(88.2%)存在t(15;17)(q22;q21)易位,其中107例(78.7%)为单纯t(15;17)(q22;q21)易位,13例(9.6%)为伴t(15;17)(q22;q21)异位的复杂核型异常;在16例无t(15;17)(q22;q21)易位的APL患者中1例(0.7%)为t(5;17)(q24;q21)易位,3例(2.2%)正常核型,12例(8.8%)未见分裂相。在所有66例进行FISH检测的APL患者中,64例存在PM I/RARα融合基因,其阳性率为97.0%,灵敏度显著高于常规染色体核型分析(p=0.041);而5例其他类型AML患者和5例正常标本均未检出PM I/RARα融合基因。结论:常规染色体核型分析和间期荧光杂交技术联合分析APL患者细胞遗传学特征是诊断该病和监测微小残留病的有力工具。
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| 关 键 词： | 急性早幼粒细胞白血病 细胞遗传学 间期荧光原位杂交 |
Comparison of conventional cytogenetics and interphase fluorescence in situ hybridization in diagnosis of acute promyelocytic leukemia |
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| Wang R,Miao KR,Qiu HR,Qian SX,Hong M,Qiao C,Zhag JF,Fan L,Wu HX,Lu H,Qiu HX,Chen LJ,Zhag SJ,Xu W,Liu P,Li JY.Comparison of conventional cytogenetics and interphase fluorescence in situ hybridization in diagnosis of acute promyelocytic leukemia[J].Journal of Experimental Hematology,2011,19(4):983-986. |
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| Authors: | Wang Rong Miao Kou-Rong Qiu Hai-Rong Qian Si-Xuan Hong Ming Qiao Chun Zhag Jian-Fu Fan Lei Wu Han-Xing Lu Hua Qiu Hong-Xia Chen Li-Juan Zhag Shu-Jiang Xu Wei Liu Peng Li Jian-Yong |
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| Institution: | WANG Rong,MIAO Kou-Rong,QIU Hai-Rong,QIAN Si-Xuan,HONG Ming,QIAO Chun,ZHAG Jian-Fu,FAN Lei,WU Han-Xing,LU Hua,QIU Hong-Xia,CHEN Li-Juan,ZHAG Shu-Jiang,XU Wei,LIU Peng,LI Jian-Yong Department of Hematology,Nanjing Medical University First Hospital,Jiangsu Provincial Hospital,Nanjing 210029,Jiangsu Province,China |
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| Abstract: | The aim of this study was to explore cytogenetic characteristics of acute promyelocytic leukemia(APL) and compare the interphase fluorescence in situ hybridization(I-FISH) with conventional cytogenetic(CC) analysis.A total number of 157 APL patients were recruited in this study,and the I-FISH and CC were applied to analyze cytogenetic features.Chromosome samples of bone marrow cells were prepared by short-term culture.Out of all 157 cases,136 were observed with CC assay,66 with I-FISH,of which 45 samples we... |
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| Keywords: | acute promyelocyte leukemia cytogenetics Interphase fluorescent in situ hybridization |
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