猪小肠粘膜下层作为组织工程肌腱支架的试验研究

目的:制备小肠粘膜下层,用作肌腱替代物,观察其替代肌腱的过程和结果,检验体内组织工程理论的可行性。方法:取猪小肠的空肠部分,经化学处理后,将小肠粘膜下层卷曲成轴,取15只19天的罗曼鸡胚趾深屈肌腱,用酶消化方法体外分离培养肌腱细胞,并进行传代扩增。对各代肌腱细胞的活性与手术中进行比较。分别于术后第3、6、9周作为时间点,每个时间点处死8只动物切取左趾移植物,进行检测,所得数据进行处理和分析。结果:SIS替代肌腱的过程是代谢过程,经过16周,SIS为宿主肌腱完全替代,其组织结构和力学性能与正常肌腱极其接近。结论:体内组织工程理论至少在肌腱再生方面是可行的。复合细胞的SIS肌腱替代物优于单纯SIS替代肌腱的作用,SIS具有很好的生物相容性和力学强度,有可能成为肌腱组织工程的一种理想的支架材料。

Pig Intestinal Submucosa as Tissue Engineering Scaffolds.Experimental Study of Tendon

Objective:To observe its replacement hamstring tendon process and result,and inspection body tissue engineering theory is feasible of small intestinal submucosa,used for preparation of substitute.Methods:Taking pork small intestines,via the jejunum part after chemical treatment,small intestinal submucosa,curled up into a shaft of 19 days only take 15 Roman chicken embryos flexor tendon,toe deep enzyme digestion methods in vitro separation,and cultivating tendon cells nestin) amplification.Each generation of...