| 正交实验法优选金银花中绿原酸提取工艺的研究 |
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| 引用本文： | 邢俊波,李萍,刘云. 正交实验法优选金银花中绿原酸提取工艺的研究[J]. 时珍国医国药, 2002, 13(6): 321-323 |
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| 作者姓名： | 邢俊波 李萍 刘云 |
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| 作者单位： | 1. 中国人民解放军总后勤部卫生部药品仪器检验所,北京100071
2. 中国药科大学,江苏,南京,210038 |
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| 基金项目： | 国家自然科学基金重点项目 (No.3 973 0 5 0 0 ) |
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| 摘 要： | 目的：优选金银花中绿原酸提取最佳工艺。方法：以绿原酸含量为考察指标，通过对冷浸、渗漉、水提石灰乳沉、热回流4种方法进行比较，再以正交实验进行优化。结果：金银花中绿原酸的最佳提取工艺为：10倍量70％乙醇，提取2次，2h/次。用醋酸乙酯萃取金银花浸膏，可以显著提高干膏中绿原酸的含量。结论：该实验有效成分提取率高，稳定性好。
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| 关 键 词： | 金银花 绿原酸 正交实验 提取工艺 |
| 文章编号： | 1008-0805(2002)06-0321-03 |
| 修稿时间： | 2002-01-07 |
Study on Optimum Extraction Process for Chlorogenic Acid in Flos Lonicerae by Orthogonal Design |
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| XING Jun bo ,LI Ping ,LIU Yun. Study on Optimum Extraction Process for Chlorogenic Acid in Flos Lonicerae by Orthogonal Design[J]. Lishizhen Medicine and Materia Medica Research, 2002, 13(6): 321-323 |
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| Authors: | XING Jun bo LI Ping LIU Yun |
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| Affiliation: | XING Jun bo 1,LI Ping 2,LIU Yun 1 |
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| Abstract: | Objective:To study the optimum extraction process for chlorogenic acid in Flos Lonicerae . Methods:The extraction process was investigated by soaking, permeating, extracting with water and calcium hydroxide, and hot refluxing, with extractive yield and chlorogenic acid as the indexes. The above processes determined were then optimized by orthogonal design. Results: The optimum extraction process consists of adding 10 folds of 70% ethanol, refluxing for 2 times, 2 h each time, and extracting with ethyl acetate finally. Conclusion:The extraction process shows high yield rate of active constituents and good stability. |
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| Keywords: | Flos Lonicerae Extraction process Orthogonal design |
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