| HPLC法测定山绿茶药材及其制剂中冬青素A的含量 |
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| 引用本文： | 魏香兰,陈筱清,杨杰,王强.HPLC法测定山绿茶药材及其制剂中冬青素A的含量[J].西北药学杂志,2010,25(3):175-176. |
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| 作者姓名： | 魏香兰 陈筱清 杨杰 王强 |
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| 作者单位： | 中国药科大学,江苏,南京,211198 |
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| 摘 要： | 目的建立山绿茶药材及其制剂中冬青素A的含量测定的方法。方法采用反相高效液相色谱法。色谱柱为C18柱(150mm×6 mm,5μm);流动相为乙腈-0.1%甲酸水(63∶37);检测波长为210 nm;流速为1.0 mL.min-1;柱温25℃;进样量20μL。结果冬青素A在0.059~1.18 g.L-1范围内与峰面积呈良好线性关系(r=0.999 6),平均回收率为100.4%(RSD为1.3%,n=6)。结论该方法可用于山绿茶药材及其制剂的质量控制,该法简便、准确、可靠。
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| 关 键 词： | 山绿茶 冬青素A HPLC |
Determination of the content of ilexgeninA in Shanlucha crude drug and its preparation |
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| WEI Xianglan,CHEN Xaoqing,YANG Jie,WANG Qiang.Determination of the content of ilexgeninA in Shanlucha crude drug and its preparation[J].Northwest Pharmaceutical Journal,2010,25(3):175-176. |
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| Authors: | WEI Xianglan CHEN Xaoqing YANG Jie WANG Qiang |
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| Institution: | (China Pharmaceutical University, Nanjing 211198,China) |
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| Abstract: | Objective To establish an HPLC method for quantitative determination of ilexgenin A in the crude drug of shanlticha and its preparations. Method RP-HPLC method with C18 column （150 mm × 6 mm, 5μm） was used and the mobile phase consisted of acetonitrile-0. 1% formic acid solution （63 ： 37）. The detection wave length was 210 nm. The flow rate was 1.0 mL· min^-1 and the column temperature was maintained at 25 ℃. The injection volume was 20 μL. Results The linear range of ilexgenin A was 0.059 1.18 g ·L^-1（ r = 0. 9996） and the mean recovery of ilexgenin A was 100. 4% （ RSD= 1.3 ~, n = 6 ）. Conclusion The proposed method was validated to be sample, accurate and reliable. |
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| Keywords: | HPLC |
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