慢性粒细胞白血病p53基因功能检测

目的：通过以ｐ２１作为标志物的功能检测方法了解慢性粒细胞白血病中ｐ５３基因功能状况。方法：采用ｗｅｓｔｅｒｎ ｂｌｏｔ方法检测未经阿霉素诱导及阿霉素诱导后慢粒患者白血病细胞中ｐ５３及ｐ２１蛋白表达。结果；对２５例慢性患者白血病细胞进行了检测，显示５例ｐ５３基因功能异常，其中１例为慢性期，显示ｐ５３基因为野生型，但无功能；１例加速期；３例急变期，分别为急粒变，急单变和多克隆急变，在急淋变中未见。

Functional detection of the p53 gene in chronic myeloid leukemia

Objective:To assess endogenous p53 status in Chronic myeloid leukemia (CML),we established a functional assay by use of p21 as a marker. Methods:Western blot was used to detect p53 and p21 protein expression of the CML cells treated with or without the chemotherapeutic drug adriamycin.Results:Samples from 25 CML patients were assessed.We identified p53 gene functional abnormalities in 5 cases,1case in chronic phase,which showed p53 gene was wild type,but was non functional;1 case in accelerated phase;3 cases in blast crisis,including 2 myeloid blast crisis and 1 mixed blast crisis. No p53 functional abnormalities was found in lymphoid blast crisis.Patients with p53 functional abnormalities had poor prognosis.Conclusions:The results present further evidence that p53 gene functional abnormalities are involved in the pathogenesis of the myeloid progression of CML and are closely related with the prognosis of the disease.There may be p53 suppressor factors or combination proteins which lead to functional inactivation of the p53 gene and it might be one of the mechanisms underlying disease progression of CML.