胃癌组织中环氧合酶-2表达与其临床病理因素及催化产物的关系

目的检测环氧合酶-2(Cyclooxygenase-2,COX-2)蛋白在胃癌组织中的表达,探讨COX-2表达与胃癌临床病理因素及其催化产物PGE2、TXA2的关系。方法应用免疫组织化学技术及放射免疫分析法检测胃癌组织中COX-2蛋白表达及PGE2、TXA2含量。结果COX-2在62.2％胃癌组织中表达增高,COX-2表达与胃癌病灶大小、淋巴结转移和TNM分期密切相关(P<0.05)。COX-2阳性表达的胃癌组织中PGE2水平明显升高,而TXB2水平在各组间差异均无显著性。结论 胃癌组织中COX-2表达增加与肿瘤大小、淋巴结转移、TNM分期有关,COX-2表达与其产物PGE2水平密切相关,COX-2可能通过催化PGE2的合成而在胃癌形成中起作用。

Correlation of expression of COX-2 to clinical pathological characters and its catalytic products in gastric carcinoma

Objective To test the expression of cyclooxygenase-2 (COX-2) in gastric carcinoma and to investigate the relationship between the expression of COX-2 and clinical pathological characters, and its relation with its catalytic products, prostaglandin E2 (PGE2 ) and thromboxane A2 (TXA2). Methods The expression of COX-2 was examined by immunohistochemical staining and the levels of PGE2 and TXB, were examined using a ra-dioimmunoassay. Results COX-2 expression was detected in 62.2% of gastric carcinoma by immunohistochemical staining with COX-2 specific polyclonal antibodies. There was a close correlations between COX-2 expression and clinicopathological factors including tumor size ( P < 0.05), lymph node metastasis (P < 0.01) and TNM stage (P < 0.05) . The cases with COX-2 expression showed significantly higher levels of PGE2 in the cancer tissues than in the normal gastric mucosa, and the levels of PGE2 in the cancer tissues with COX-2 expression were higher than in those without. The levels of TXB2 was not different between the normal and cancer tissue specimens in the patients with COX-2 expression or those without. Conclusion COX-2 overexpression was associated with tumor size, lymph node metastasis and TNM stage. Increased levels of PGE2 in the gastric carcinoma tissues were prominent in patients with COX-2 expression, so the role that COX-2 plays in the progression of gastric carcinoma may be associated with PGE2 biosynthesis.