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Transganglionic regulation of central terminals of dorsal root ganglion cells by nerve growth factor (NGF)
Authors:Bert Csillik   Martin E. Schwab  Hans Thoenen
Affiliation:1. New York University Silver School of Social Work, 1 Washington Square North, New York, NY 10003, United States;2. Silberman School of Social Work at Hunter College, 2180 Third Avenue @ 119th St., New York, NY 10035, United States;3. Virginal Commonwealth University Department of Psychology, 806 West Franklin Street, Richmond, Virginia 23284-2018, United States;4. Services for the UnderServed, Inc., 305 Seventh Avenue, 10th Floor, New York, NY 10001, United States;5. New York University Rory Meyers College of Nursing, 433 First Avenue, 7th Floor, New York, NY 10010, United States;1. Gynecologic Cancer Center of Excellence, Department of Gynecologic Surgery and Obstetrics, Uniformed Services University and Walter Reed National Military Medical Center, 8901 Wisconsin Avenue, Bethesda 20889, MD, USA;2. Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc. Bethesda, MD 20817, USA;3. Department of Obstetrics, Gynecology and Reproductive Biology, College of Human Medicine, Michigan State University Grand Rapids, MI, USA;4. The American Genome Center, Department of Anatomy Physiology and Genetics, Collaborative Health Initiative Research Program, Uniformed Services University, 4301 Jones Bridge Road, Bethesda, MD 20814, USA;5. The John P. Murtha Cancer Center, Uniformed Services University and Walter Reed National Military Medical Center, 8901 Wisconsin Avenue, Bethesda 20889, MD, USA;6. Departments of Radiation Biology and Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA;7. Department of Obstetrics and Gynecology, Inova Fairfax Medical Campus, 3300 Gallows Rd. Falls Church, VA 22042, USA.;1. Department of Orthopedics, Shanghai Tenth People''s Hospital, Tongji University School of Medicine. No.301 Yanchang Middle Road, Shanghai 200072, China;2. Department of Orthopedics, Shanghai East Hospital, Tongji University School of Medicine, China. No. 150 Jimo Road, Shanghai 200120, China;3. Department of Bone and Soft Tissue Tumors, Tianjin Medical University Cancer Institute and Hospital. No.1 North Huanhu West Road, Tianjin 300060, China
Abstract:Blockade of axonal transport or transection of the rat sciatic nerve results in transganglionic degenerative atrophy (TDA) of nerve terminals containing fluoride-resistant acid phosphatase (FRAP) in the Rolando substance of the spinal cord. Application of vinblastine (9 micrograms) in a cuff around the sciatic nerve of adult rats blocked the retrograde transport of [125I]NGF in sensory fibers; this amount of vinblastine is identical to the threshold amount that induces TDA. Conversely, application of NGF to the proximal stump of the transected sciatic nerve prevented or delayed the occurrence of TDA as reflected by the maintenance of FRAP in the upper dorsal horn, that otherwise would inevitably disappear following the peripheral nerve lesion. These results suggest that endogenous NGF transported retrogradely in peripheral sensory fibers of the adult rat under normal conditions may be responsible for the regulation of the structural and functional integrity of the central terminals of these FRAP-containing primary sensory neurons and that TDA may be the consequence of the failure of NGF to reach the perikarya of these neurons.
Keywords:nerve growth factor   spinal cord   axoplasmic transport   dorsal root ganglion   degeneration
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