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| 血管内皮细胞线粒体膜电位与缺氧及血管紧张素Ⅱ的相关性 | |
| 引用本文： | 刘玉,胡建林,李鸣皋,马贵喜,韩磊,黄友章.血管内皮细胞线粒体膜电位与缺氧及血管紧张素Ⅱ的相关性[J].临床军医杂志,2008,36(4). |
| 作者姓名： | 刘玉 胡建林 李鸣皋 马贵喜 韩磊 黄友章 |
| 作者单位： | 1. 解放军第三军医大学附属西南医院,呼吸科,重庆,400038 2. 解放军海军总医院,海军航空潜水医学中心,北京,100037 3. 解放军海军总医院,血液科,北京,100037 |
| 摘 要： | 目的观察缺氧及血管紧张素Ⅱ(Ang-Ⅱ)对血管内皮细胞(VEC)线粒体膜电位的影响。方法建立VEC缺氧损伤模型;实验设为空白对照组、缺氧组、Ang-Ⅱ作用组、缺氧加Ang-Ⅱ作用组等四组。细胞经过Rhodamine 123负载后,用激光共聚焦显微镜测定VEC内Rhodamine 123的荧光强度代表线粒体膜电位。结果VEC分别经过缺氧及Ang-Ⅱ损伤后,线粒体膜电位明显降低(P<0.01);缺氧与Ang-Ⅱ联合作用可引起VEC线粒体膜电位较单纯缺氧或单纯Ang-Ⅱ作用进一步降低(P<0.05)。结论缺氧及Ang-Ⅱ可引起VEC线粒体膜电位明显降低,造成VEC损伤。 |
| 关 键 词： | 缺氧 血管紧张素Ⅱ 线粒体膜电位 血管内皮细胞 |
Correlation of Mitochondria Membrane Potential of Vascular Endothelial Cells to Hypoxia and Angiotesin |
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| Liu Yu,Hu Jian-lin,Li Ming-gao,Ma Gui-xi,Han Lei,Huang You-zhang.Correlation of Mitochondria Membrane Potential of Vascular Endothelial Cells to Hypoxia and Angiotesin[J].Clinical Journal of Medical Officer,2008,36(4). | |
| Authors: | Liu Yu Hu Jian-lin Li Ming-gao Ma Gui-xi Han Lei Huang You-zhang |
| Institution: | Liu Yu1,Hu Jian-lin1,Li Ming-gao2,Ma Gui-xi2,Han Lei2,Huang You-zhang3 (1. Department of Respiratory Medicine,Southwest Hospital of Third Military Madical University,Chongqing 400038,China,2. Aviation & Diving Medical Center,3. Department of Hematology,PLA Navy General Hospital,Beijing 100037,China) |
| Abstract: | Objective To study the effect of hypoxia and angiotesinⅡ (AngⅡ) on mitochondria membrane potential (MMP) of vascular endothelial cells (VECs). Methods Hypoxia-induced injury model of VECs was established, and then cultured VECs were divided into four groups: control group, simple hypoxia group, simple AngⅡ group, hypoxia+AngⅡ group, which were loaded with Rhodamine 123. MMP was detected by laser confocal microscopy to see if there were any differences among the groups. Results Compared with control group, decrease in MMP was observed either in the hypoxia group or in the Ang Ⅱ group (P<0.01). This change was clearer in the hypoxia+ AngⅡ group than in any of the other groups (P<0.05). Conclusion Hypoxia or AngⅡ could significantly decrease MMP of VECs, and injure VECs. |
| Keywords: | hypoxia angiotesinⅡ mitochondria membrane potential vascular endothelial cell |
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