阿维A治疗前后对银屑病患者血管内皮生长因子表达的影响

目的 研究阿维A对血管内皮生长因子(VEGF)促银屑病血管生成的调节作用,探讨阿维A治疗银屑病的机制。方法 免疫组化法检测32例银屑病患者经阿维A治疗前后皮损的微血管密度和VEGF蛋白的表达情况。应用双抗体夹心酶联免疫吸附法(ELISA)检测32例银屑病患者经阿维A治疗前后外周血清中VEGF水平。结果 ①银屑病患者经阿维A治疗前皮损VEGF蛋白的表达及微血管密度值显著高于治疗后(P<0.05)和正常人对照组(P<0.001)。②银屑病患者阿维A治疗前的血清VEGF水平高于治疗后(P<0.001)及正常人对照组(P<0.001)。结论 VEGF在银屑病新生血管生成中起重要作用。阿维A可能通过抗新生血管生成的作用来治疗银屑病。

Effects of Acitretin on Angiogenesis induced by Vascular Endothelial Growth Factor (VEGF) in Patients with Psoriasis

Objective To investigate the mechanism of effects of acitretin on the treatment of psoriasis. Methods The expression of vascular endothelial growth factor (VEGF) and micro-vascular density (MVD) were detected by immunohistochemical technique before and after treatment with acitretin in 32 patients with psoriasis. Serum level of VEGF was measured by double antibody sandwich enzyme-linked immunosorbent assay (ELISA). Results The expression of VEGF protein and MVD were significantly higher in psoriatic lesions before treatment with acitretin than those after treatment (P<0.05) and those in the normal controls (P<0.001). Conclusions VEGF plays an important role in the angiogenesis of psoriasis, which indicates that anti-angiogenesis may be involved in the mechanism of effects of acitretin on the treatment of psoriasis.