| 白纹伊蚊感染登革2型病毒后基因表达变化的研究 |
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| 引用本文： | 杨发青,赵彤言,谢超,朱礼华,陆宝麟. 白纹伊蚊感染登革2型病毒后基因表达变化的研究[J]. 寄生虫与医学昆虫学报, 2004, 11(2): 83-86 |
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| 作者姓名： | 杨发青 赵彤言 谢超 朱礼华 陆宝麟 |
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| 作者单位： | 军事医学科学院卫生学环境医学研究所;军事医学科学院流行病学与微生物学研究所,北京,100071 |
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| 基金项目： | 中国人民解放军总后勤部“九五”杰出中青年基金 |
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| 摘 要： | 白纹伊蚊感染登革 2型病毒 2 4h后 ,和对照蚊虫一起提取RNA ,用差异显示PCR (DDRT PCR)技术对蚊虫感染病毒后基因表达的变化进行研究。白纹伊蚊感染登革 2型病毒后 ,经过 8条随机引物和 3条 3′端锚定引物配对扩增 ,发现了 6条表达有差异的片段 ,其中 5条为感染后表达量增加的片段 ,1条为感染后表达量减少的片段 ,并对这些片段进行了克隆和测序 ,通过GenBank查询 ,其中 5条为未知序列 ,1条表达量增高的片段和果蝇核糖体蛋白S5基因有较高的同源性。这些差异片段的发现可能对探讨媒介蚊虫对登革病毒的易感性机理有意义
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| 关 键 词： | 白纹伊蚊 登革2型病毒 DDRT-PCR 核糖体蛋白S5基因 |
| 修稿时间： | 2003-10-30 |
STUDIES ON DIFFERENTIAL EXPRESSED GENES OF AEDES ALBOPICTUS ORALLY INFECTED WITH DENGUE TYPE 2 VIRUS |
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| YANG Fa-Qing ZHAO Tong-Yan XIE Chao ZHU Li-Hua LU Bao-Lin. STUDIES ON DIFFERENTIAL EXPRESSED GENES OF AEDES ALBOPICTUS ORALLY INFECTED WITH DENGUE TYPE 2 VIRUS[J]. Acta Parasitologica et Medica Entomologica Sinica, 2004, 11(2): 83-86 |
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| Authors: | YANG Fa-Qing ZHAO Tong-Yan XIE Chao ZHU Li-Hua LU Bao-Lin |
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| Abstract: | Differential expressed genes of Aedes albopictus was studied by DDRT-PCR technique,24h after mosquito infected with dengue type 2 virus.Six differential expressed gene fragments,including of five upregulated and one downregulated,were found by DDRT-PCR which amplified with 8 random primers and 3 anchor primers.It is found that one of upregulated gene fragment was identified as ribosomal protein S5 gene,after DNA cloning and sequencing analyses.The others were unknown gene fragments.The result would be significant for further understanding the mechanism of susceptibility of Ae.albopictus to infection with dengue virus. |
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| Keywords: | Aedes albopictus Dengue type 2 virus DDRT-PCR Ribosomal protein S5 gene |
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