T7噬菌体展示技术筛选丙型肝炎病毒非结构蛋白3的相互作用蛋白

目的 用T7噬菌体筛选系统筛选丙型肝炎病毒非结构蛋白（NS）3的相互作用蛋白。方法 应用T7噬菌体展示技术，以通过原核表达的方式得到的丙型肝炎病毒NS3为靶分子，对噬菌体人肝细胞cDNA文库进行筛选，对筛选到的克隆进行DNA序列分析及同源性研究。结果 经鉴定得到2个阳性克隆，并确定能与丙型肝炎病毒NS3相互作用的蛋白质为serine peptidase inhibitor，cladeA，member 1（SERPINA1）和cyclophilin-LC。结论 T7噬菌体筛选系统是筛选相互作用蛋白的一种简单、快速和有效的手段，筛选到的相互作用蛋白为进一步探讨NS3的致病、致癌机制提供了重要依据。

Screening of proteins interacting with hepatitis C virus NS3 from T7-phage display library

Objective To screen and identify proteins that interact with hepatitis C virus NS3 by means of T7-phage display system. Method Hepatitis C virus NS3 was expressed by prokaryotic expression and used as a selected molecule to biopan the T7 select human liver cDNA library; the selected positive clones were identified using DNA sequencing and analyzed with BLAST program in GenBank. Result After BLAST analysis in all the positive clones, the proteins which interacted with the hepatitis C virus NS3 were found to be serpin peptidase inhibitor, clade A, member 1 (SERPINA1) and cyclophilin-LC. Conclusion T7-phage display system is a convenient, rapid and effective method for screening interacting proteins. The proteins thus selected will provide an important means for studying the pathogeuesis and carcinogenesis of HCV.