Naloxone and flumazenil fail to antagonize the isoflurane-induced suppression of dorsal horn neurons in cats

Effects of naloxone and flumazenil on isoflurane activities were examined on dorsal horn neurons in cats. Isoflurane suppressed bradykinin-induced nociceptive responses in transected feline spinal cords. The bradykinin-induced neuronal firing rates were significantly suppressed by 60.0%, 35.3% and 32.2% at 10, 20 and 30min after isoflurane administration, respectively. The 32.3% suppression on bradykinin-induced neuronal responses at 30min after isoflurane administration was not reversed 5min after administration of naloxone (36.4% suppression). The suppressive effects of isoflurane were not reversed by naloxone (0.2mg·kg^–1, i.v.). Similarly, the benzodiazepine antagonist, flumazenil (0.2mg·kg^–1, i.v.), did not affect the suppressive effects of isoflurane. Failure of naloxone and flumazenil to reverse the suppressive effects of isoflurane suggests that isoflurane interacts with neither opioid nor benzodiazepine receptors in producing its suppressive action on nociceptive responses in dorsal horn neurons of the feline spinal cord.(Okuda T, Wakita K, Tsuchiya N, et al.: Naloxone and flumazenil fail to antagonize the isoflurane-induced suppression of dorsal horn neurons in cats. J Anesth 7: 462–467, 1993)