成人睾丸支持细胞的分离培养及其免疫豁免机制

目的：建立成人睾丸支持细胞的体外培养方法并探讨其免疫豁免机制。方法：依次用胰蛋白酶、胶原酶、透明质酸酶及脱氧核糖核酸酶消化制备成人睾丸支持细胞，以SABC染色法检测支持细胞上Fas-L和TGF-β1的表达。将其与成人脾细胞共同培养，用MTT比色法检测其对脾细胞增殖的抑制作用。结果：成人睾丸支持细胞占培养细胞总数的80％以上，活性可达90％。睾丸支持细胞上可表达Fas-L和TGF-β1，体外可抑制共培养的脾细胞增殖。结论：建立了培养成人睾丸支持细胞的方法，其免疫豁免机制可能与Fas-L和TGF-β1的表达有关。

The isolation and culture of Sertoli cells from adult testis and their immune privilege mechanism

AIM: To set up a method of isolation and culture of Sertoli cells from adult testis and to investigate their immune privilege mechanism. METHODS: Adult testis Sertoli cells were prepared successfully by digestion with trypsin, collagenase, hyaluronidase and DNase, and then the expression of Fas-L and TGF-beta1 was examined by SABC staining. The Sertoli cells were cocultured with adult splenocytes to detect the inhibitory effect of Sertoli cells on splenocyte proliferation by MTT colorimetry. RESULTS: In cultured cells, Sertoli cells accounted for more than 80% of total cells. The activity of Sertoli cells reached 90%. Sertoli cells expressed Fas-L and TGF-beta1 and could inhibit the proliferation of cocultured splenocytes in vitro. CONCLUSION: A method of isolation and culture of Sertoli cells from adult testis has been established. The immune privilege mechanism of Sertoli cells may be related to the expression of Fas-L and TGF-beta1.