几种肿瘤转移基因变化与卵巢癌转移的相关性

目的　研究 MTA1、nm2 3H1 及 E- Cadherin(E- cad)的表达及突变与卵巢癌转移的相关性。方法　应用 RT- PCR、RT- PCR- SSCP和免疫组化技术 ,对正常卵巢、卵巢癌及其配对淋巴结组织进行 MTA1、nm2 3H1m RNA表达和突变及 E- cad蛋白表达的检测。结果　有转移的卵巢癌原发灶 MTA1m RNA高表达率为 10 0 % (7/7) ,无转移为 38.5 % (5 / 13) ,P=0 .0 10 3。有癌转移淋巴结高表达率为 87.5 % (6 / 7) ,而无癌转移为 2 3% (3/ 13) ,P=0 .0 118。有转移卵巢癌原发灶 nm2 3H1 m RNA低表达率为 10 0 % (7/ 7) ,无转移为 30 % (4/ 13) ,P=0 .0 0 43。有癌转移淋巴结低表达率为 10 0 % (7/ 7) ,无癌转移为 38.5 % (5 / 13) ,P=0 .0 10 2。无转移卵巢癌原发灶 E- cad蛋白表达阳性率为 46 .2 % (6 / 13) ,而有转移者 7例均无阳性表达 ,表达率为 0 ,P=0 .0 44。三基因表达相关性分析表明 ,MTA1与 nm2 3H1 、C- cad呈负相关 (r=- 0 .90 3、- 0 .80 2 ) ,nm2 3H1 与 E- cad呈正相关 (r=0 .72 4)。SSCP分析未发现 MTA1和 nm2 3H1 基因突变。结论　 MTA1及 nm2 3H1 、E- cad的表达与卵巢癌转移呈正、负相关关系 ,起着正、负调控的重要作用。这三个基因的异常表达是卵巢癌转移中的频发事件而与基因突变无关

Alteration of Multiple Tumor Metastatic Genes with Correlation to Metastasis in Ovarian Carcinoma

Objective To investigate the expression and mutation of MTA1? nm23H 1 and E cadherin(E cad) genes in ovarian carcinoma (OC) in relation to lymph node (LN) metastasis. Methods A panel of normal ovarian tissues, primary OC specimens and corresponding LNS was examined for mRNA expression and mutation of MTA1 and nm23H 1 and protin expression of E cad genes by using RT PCR, RT PCR SSCP and immunohistochemistry. Results The frequency of MTA1 over expression was 100%(7/7)in primary OC with metastasis but only 38.5%(5/13) in those without metastasis( P =0.0103). Overexpression of MTA1 was observed in 87.5%(6/7) of LNS with metastasis but in only 23%(3/13) of LNS without metastasis ( P =0.0118). In contrast with MTA1, low expression of nm23H 1 mRNA was seen in 7 of 7 OC with metastasis but only in 4 of 13(30%) of those without metastasis ( P =0.0043). Low nm23H1 expression was also seen in 7 of 7 LNS with metastasis but only in 5 of 13(38.5%) nonmetastatic LNS ( P =0.0102).Meantime, no expression of E cad protein was observed in 7 of 7 OC with metastasis but in 6 of 13(46.2%)of those without metastasis ( P =0.044). In correlation analysis of the three genes, MTA1 reversely correlated with nm23H 1 and E cad respectively ( r = -0 903, -0.803), and positive correlation existed between nm23H 1 and E cad (r=0.724).No mutation of MTA1, nm23H 1 and was found by SSCP analysis. Conclusion The mRNA expression of MTA1, nm23H 1 and E cad is positively and negatively correlated with LN metastasis. The expression abnormalities but not the mutations of the three genes are frequent events related to LN metastasis of ovarian cancer.