与骨髓MSC共孵育的T淋巴细胞对白血病细胞的杀伤作用

目的 探讨经MSC诱导免疫耐受的T淋巴细胞对与MSC同源的肿瘤细胞的杀伤活性，以证实MSC诱导免疫耐受的同时，是否也削弱了移植物抗肿瘤的作用。方法 初治的白血病患者留取原代肿瘤细胞，液氮保存，诱导缓解后培养同一患者的骨髓MSC，取异体T淋巴细胞与MSC共孵育，比较与MSC共孵育的T淋巴细胞对MSC同源的肿瘤细胞的杀伤作用是否减弱。结果 与MSC共孵育后，T淋巴细胞对MSC同源的肿瘤细胞仍有杀伤作用，但与未共孵育者相比。杀伤活性减弱。结论 MSC在诱导免疫耐受的同时也削弱了GVI，的作用，提示在以MSC诱导免疫耐受预防GVHD的方案中，应考虑同时加强GVL作用，以防肿瘤复发。

Effects of bone marrow derived mesenchymal stem cells on the T cell killing activity of primary leukemic cells

Objective To evaluated the killing activity of T cell which is co- cultured with patient bone marrow derived mesenchymal stem cells (MSCs) on the same patient primary leukemic cells. Methods Primary leukemic cells were deepfreezed when the patient were diagnosis. When the patient were induced complete remission, the patient's bone marrow derived MSCs were expanded and co- cultured with allogenic T cells. The killing activity of T cells co- cultured with or without MSCs on the primary leukemic cells were compared. Results Compared with the T cells without co- culture with the MSCs, the killing activity on the same origin with the MSCs' primary leukemic cells of T cells co- cultured with MSCs was decreased but still exist. Conclusions The activity of the graft versus leukemia effect ( GVL) was decreased meanwhile immunological tolerance induced by bone marrow- derived mesenchymal stem cells. It indicated that when the immunological tolerance induced by bone marrow- derived mesenchymal stem cells clinically, the GVL should be considered to avoid the relapsed of the leukemia.